On the Mechanism of Integration of Transforming Deoxyribonucleate
The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers 32P as a radioactive label a...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1966
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195541/ |
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pubmed-2195541 |
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oai_dc |
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pubmed-21955412008-04-23 On the Mechanism of Integration of Transforming Deoxyribonucleate Fox, Maurice S. Genetic Transformation The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers 32P as a radioactive label and 2H and 15N as density labels has permitted the characterization of the product of recombination between the newly introduced DNA and the DNA of a recipient bacterium. The evidence for a single strand displacement mechanism producing a hybrid structure in the DNA of the recipient bacteria is presented. Progeny of single transformants have been examined. The results of these segregation studies permit the further characterization of this hybrid product of transformation as a genetically heterozygous structure. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2195541/ /pubmed/4381781 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Fox, Maurice S. |
| spellingShingle |
Fox, Maurice S. On the Mechanism of Integration of Transforming Deoxyribonucleate |
| author_facet |
Fox, Maurice S. |
| author_sort |
Fox, Maurice S. |
| title |
On the Mechanism of Integration of Transforming Deoxyribonucleate |
| title_short |
On the Mechanism of Integration of Transforming Deoxyribonucleate |
| title_full |
On the Mechanism of Integration of Transforming Deoxyribonucleate |
| title_fullStr |
On the Mechanism of Integration of Transforming Deoxyribonucleate |
| title_full_unstemmed |
On the Mechanism of Integration of Transforming Deoxyribonucleate |
| title_sort |
on the mechanism of integration of transforming deoxyribonucleate |
| description |
The characteristics of the intermediates in the reaction, between DNA and pneumococcus, that results in genetic transformation are described in so far as they have been characterized. Transformation with DNA isolated from bacteria carrying in addition to genetic markers 32P as a radioactive label and 2H and 15N as density labels has permitted the characterization of the product of recombination between the newly introduced DNA and the DNA of a recipient bacterium. The evidence for a single strand displacement mechanism producing a hybrid structure in the DNA of the recipient bacteria is presented. Progeny of single transformants have been examined. The results of these segregation studies permit the further characterization of this hybrid product of transformation as a genetically heterozygous structure. |
| publisher |
The Rockefeller University Press |
| publishDate |
1966 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195541/ |
| _version_ |
1611432112798302208 |