The Structure and Function of the DNA from Bacteriophage Lambda
The position and orientation of genes in lambda and lambda dg DNA are described. The position of six genes located in the right half of isolated lambda DNA was found to be -(N, i λ)--O-P---Q-R-(right end of DNA), which is their order on the genetic map of the vegetative phage. The order of the thre...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1966
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195532/ |
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pubmed-2195532 |
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pubmed-21955322008-04-23 The Structure and Function of the DNA from Bacteriophage Lambda Hogness, David S. DNA and Alterations of DNA The position and orientation of genes in lambda and lambda dg DNA are described. The position of six genes located in the right half of isolated lambda DNA was found to be -(N, i λ)--O-P---Q-R-(right end of DNA), which is their order on the genetic map of the vegetative phage. The order of the three genes of the galactose operon (k, t, and e) located in the left half of lambda dg DNA was found to be (left end of DNA)----k-t-e-, consistent with Campbell's model (5) for the formation of this variant. Gene orientation, defined as the direction of transcription along the DNA, is inferred to be from right to left for the galactose operon in lambda dg DNA. The strand of lambda DNA which functions as template in transcription of N, an "early" gene required for normal replication of lambda DNA, was determined as a first step in ascertaining the orientation of this gene. The method includes isolation of each strand, formation of each of two heteroduplex molecules consisting of one strand from wild-type and one from an N mutant) and comparison of their N activities. The second step, which consists of ascertaining the 5'-to-3' direction of each strand, is discussed, as is a determination of the orientation of gene R. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2195532/ /pubmed/5967430 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Hogness, David S. |
| spellingShingle |
Hogness, David S. The Structure and Function of the DNA from Bacteriophage Lambda |
| author_facet |
Hogness, David S. |
| author_sort |
Hogness, David S. |
| title |
The Structure and Function of the DNA from Bacteriophage Lambda |
| title_short |
The Structure and Function of the DNA from Bacteriophage Lambda |
| title_full |
The Structure and Function of the DNA from Bacteriophage Lambda |
| title_fullStr |
The Structure and Function of the DNA from Bacteriophage Lambda |
| title_full_unstemmed |
The Structure and Function of the DNA from Bacteriophage Lambda |
| title_sort |
structure and function of the dna from bacteriophage lambda |
| description |
The position and orientation of genes in lambda and lambda dg DNA are described. The position of six genes located in the right half of isolated lambda DNA was found to be -(N, i
λ)--O-P---Q-R-(right end of DNA), which is their order on the genetic map of the vegetative phage. The order of the three genes of the galactose operon (k, t, and e) located in the left half of lambda dg DNA was found to be (left end of DNA)----k-t-e-, consistent with Campbell's model (5) for the formation of this variant. Gene orientation, defined as the direction of transcription along the DNA, is inferred to be from right to left for the galactose operon in lambda dg DNA. The strand of lambda DNA which functions as template in transcription of N, an "early" gene required for normal replication of lambda DNA, was determined as a first step in ascertaining the orientation of this gene. The method includes isolation of each strand, formation of each of two heteroduplex molecules consisting of one strand from wild-type and one from an N mutant) and comparison of their N activities. The second step, which consists of ascertaining the 5'-to-3' direction of each strand, is discussed, as is a determination of the orientation of gene R. |
| publisher |
The Rockefeller University Press |
| publishDate |
1966 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195532/ |
| _version_ |
1611432107243995136 |