Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells

Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells

Malaria parasite species that infect mammals, including humans, must first take up residence in hepatic host cells as exoerythrocytic forms (EEF) before initiating infection of red blood cells that leads to malaria disease. Despite the importance of hepatic stages for immunity against malaria, littl...

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Main Authors: Kaiser, Karine, Camargo, Nelly, Kappe, Stefan H.I.
Format: Online
Language:English
Published: The Rockefeller University Press 2003
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193875/
id pubmed-2193875
recordtype oai_dc
spelling pubmed-21938752008-04-11 Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells Kaiser, Karine Camargo, Nelly Kappe, Stefan H.I. Brief Definitive Report Malaria parasite species that infect mammals, including humans, must first take up residence in hepatic host cells as exoerythrocytic forms (EEF) before initiating infection of red blood cells that leads to malaria disease. Despite the importance of hepatic stages for immunity against malaria, little is known about their biology and antigenic composition. Here, we show that sporozoites, the parasites' transmission stage that resides in the mosquito vector salivary glands, can transform into early EEF without intracellular residence in host hepatocytes. The morphological sequence of transformation and the expression of proteins in the EEF appear indistinguishable from parasites that develop within host cells. Transformation depends on temperature elevation to 37°C and serum. Our findings demonstrate that residence in a host hepatocyte or specific host cell–derived factors are not necessary to bring about the profound morphological and biochemical changes of the parasite that occur after its transmission from vector to mammalian host. The Rockefeller University Press 2003-04-21 /pmc/articles/PMC2193875/ /pubmed/12707302 http://dx.doi.org/10.1084/jem.20022100 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Kaiser, Karine
Camargo, Nelly
Kappe, Stefan H.I.
spellingShingle Kaiser, Karine
Camargo, Nelly
Kappe, Stefan H.I.
Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
author_facet Kaiser, Karine
Camargo, Nelly
Kappe, Stefan H.I.
author_sort Kaiser, Karine
title Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
title_short Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
title_full Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
title_fullStr Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
title_full_unstemmed Transformation of Sporozoites into Early Exoerythrocytic Malaria Parasites Does Not Require Host Cells
title_sort transformation of sporozoites into early exoerythrocytic malaria parasites does not require host cells
description Malaria parasite species that infect mammals, including humans, must first take up residence in hepatic host cells as exoerythrocytic forms (EEF) before initiating infection of red blood cells that leads to malaria disease. Despite the importance of hepatic stages for immunity against malaria, little is known about their biology and antigenic composition. Here, we show that sporozoites, the parasites' transmission stage that resides in the mosquito vector salivary glands, can transform into early EEF without intracellular residence in host hepatocytes. The morphological sequence of transformation and the expression of proteins in the EEF appear indistinguishable from parasites that develop within host cells. Transformation depends on temperature elevation to 37°C and serum. Our findings demonstrate that residence in a host hepatocyte or specific host cell–derived factors are not necessary to bring about the profound morphological and biochemical changes of the parasite that occur after its transmission from vector to mammalian host.
publisher The Rockefeller University Press
publishDate 2003
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193875/
_version_ 1611431355189559296

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