A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion

Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in a...

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Main Authors:	Murata, Makoto, Warren, Edus H., Riddell, Stanley R.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2003
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193779/

id	pubmed-2193779
recordtype	oai_dc
spelling	pubmed-21937792008-04-11 A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion Murata, Makoto Warren, Edus H. Riddell, Stanley R. Article Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in all cases, amino acid differences between homologous donor and recipient proteins due to nucleotide polymorphisms in the respective genes were responsible for immunogenicity. Here, we have used cDNA expression cloning to identify a novel human minor H antigen encoded by UGT2B17, an autosomal gene in the multigene UDP-glycosyltransferase 2 family that is selectively expressed in liver, intestine, and antigen-presenting cells. In contrast to previously defined human minor H antigens, UGT2B17 is immunogenic because of differential expression of the protein in donor and recipient cells as a consequence of a homozygous gene deletion in the donor. Deletion of individual members of large gene families is a common form of genetic variation in the population and our results provide the first evidence that differential protein expression as a consequence of gene deletion is a mechanism for generating minor H antigens in humans. The Rockefeller University Press 2003-05-19 /pmc/articles/PMC2193779/ /pubmed/12743171 http://dx.doi.org/10.1084/jem.20030044 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Murata, Makoto Warren, Edus H. Riddell, Stanley R.
spellingShingle	Murata, Makoto Warren, Edus H. Riddell, Stanley R. A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
author_facet	Murata, Makoto Warren, Edus H. Riddell, Stanley R.
author_sort	Murata, Makoto
title	A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
title_short	A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
title_full	A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
title_fullStr	A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
title_full_unstemmed	A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion
title_sort	human minor histocompatibility antigen resulting from differential expression due to a gene deletion
description	Minor histocompatibility antigens (minor H antigens) are targets of graft-versus-host disease and graft-versus-leukemia responses after allogeneic human leukocyte antigen identical hematopoietic stem cell transplantation. Only a few human minor H antigens have been molecularly characterized and in all cases, amino acid differences between homologous donor and recipient proteins due to nucleotide polymorphisms in the respective genes were responsible for immunogenicity. Here, we have used cDNA expression cloning to identify a novel human minor H antigen encoded by UGT2B17, an autosomal gene in the multigene UDP-glycosyltransferase 2 family that is selectively expressed in liver, intestine, and antigen-presenting cells. In contrast to previously defined human minor H antigens, UGT2B17 is immunogenic because of differential expression of the protein in donor and recipient cells as a consequence of a homozygous gene deletion in the donor. Deletion of individual members of large gene families is a common form of genetic variation in the population and our results provide the first evidence that differential protein expression as a consequence of gene deletion is a mechanism for generating minor H antigens in humans.
publisher	The Rockefeller University Press
publishDate	2003
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193779/
_version_	1611431297550385152

A Human Minor Histocompatibility Antigen Resulting from Differential Expression due to a Gene Deletion

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