Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells
We analyzed the interaction between human peripheral blood natural killer (NK) cells and monocyte-derived immature dendritic cells (DC). Fresh NK cells were activated, as indicated by the induced expression of the CD69 antigen, and their cytolytic activity was strongly augmented by contact with lipo...
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The Rockefeller University Press
2002
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193595/ |
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pubmed-21935952008-04-14 Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells Gerosa, Franca Baldani-Guerra, Barbara Nisii, Carla Marchesini, Viviana Carra, Giuseppe Trinchieri, Giorgio Original Article We analyzed the interaction between human peripheral blood natural killer (NK) cells and monocyte-derived immature dendritic cells (DC). Fresh NK cells were activated, as indicated by the induced expression of the CD69 antigen, and their cytolytic activity was strongly augmented by contact with lipopolysaccharide (LPS)-treated mature DC, or with immature DC in the presence of the maturation stimuli LPS, Mycobacterium tuberculosis or interferon (IFN)-α. Reciprocally, fresh NK cells cultured with immature DC in the presence of the maturation stimuli strongly enhanced DC maturation and interleukin (IL)-12 production. IL-2–activated NK cells directly induced maturation of DC and enhanced their ability to stimulate allogeneic naive CD4+ T cells. The effects of NK cells were cell contact dependent, although the secretion of IFN-γ and TNF also contributed to DC maturation. Within peripheral blood lymphocytes the reciprocal activating interaction with DC was restricted to NK cells, because the other lymphocyte subsets were neither induced to express CD69, nor induced to mature in contact with DC. These data demonstrated for the first time a bidirectional cross talk between NK cells and DC, in which NK cells activated by IL-2 or by mature DC induce DC maturation. The Rockefeller University Press 2002-02-04 /pmc/articles/PMC2193595/ /pubmed/11828007 http://dx.doi.org/10.1084/jem.20010938 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Gerosa, Franca Baldani-Guerra, Barbara Nisii, Carla Marchesini, Viviana Carra, Giuseppe Trinchieri, Giorgio |
| spellingShingle |
Gerosa, Franca Baldani-Guerra, Barbara Nisii, Carla Marchesini, Viviana Carra, Giuseppe Trinchieri, Giorgio Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| author_facet |
Gerosa, Franca Baldani-Guerra, Barbara Nisii, Carla Marchesini, Viviana Carra, Giuseppe Trinchieri, Giorgio |
| author_sort |
Gerosa, Franca |
| title |
Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| title_short |
Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| title_full |
Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| title_fullStr |
Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| title_full_unstemmed |
Reciprocal Activating Interaction between Natural Killer Cells and Dendritic Cells |
| title_sort |
reciprocal activating interaction between natural killer cells and dendritic cells |
| description |
We analyzed the interaction between human peripheral blood natural killer (NK) cells and monocyte-derived immature dendritic cells (DC). Fresh NK cells were activated, as indicated by the induced expression of the CD69 antigen, and their cytolytic activity was strongly augmented by contact with lipopolysaccharide (LPS)-treated mature DC, or with immature DC in the presence of the maturation stimuli LPS, Mycobacterium tuberculosis or interferon (IFN)-α. Reciprocally, fresh NK cells cultured with immature DC in the presence of the maturation stimuli strongly enhanced DC maturation and interleukin (IL)-12 production. IL-2–activated NK cells directly induced maturation of DC and enhanced their ability to stimulate allogeneic naive CD4+ T cells. The effects of NK cells were cell contact dependent, although the secretion of IFN-γ and TNF also contributed to DC maturation. Within peripheral blood lymphocytes the reciprocal activating interaction with DC was restricted to NK cells, because the other lymphocyte subsets were neither induced to express CD69, nor induced to mature in contact with DC. These data demonstrated for the first time a bidirectional cross talk between NK cells and DC, in which NK cells activated by IL-2 or by mature DC induce DC maturation. |
| publisher |
The Rockefeller University Press |
| publishDate |
2002 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193595/ |
| _version_ |
1611431189073100800 |