Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes

Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes

Upon activation, B lymphocytes can change the isotype of the antibody they express by immunoglobulin (Ig) isotype switch recombination. In previous studies on the regulation of human IgG expression, we demonstrated that interleukin 10 (IL-10) could stimulate IgG1 and IgG3 secretion by human CD40-act...

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Format: Online
Language:English
Published: The Rockefeller University Press 1996
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192363/
id pubmed-2192363
recordtype oai_dc
spelling pubmed-21923632008-04-16 Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes Articles Upon activation, B lymphocytes can change the isotype of the antibody they express by immunoglobulin (Ig) isotype switch recombination. In previous studies on the regulation of human IgG expression, we demonstrated that interleukin 10 (IL-10) could stimulate IgG1 and IgG3 secretion by human CD40-activated naive (sIgD+) tonsillar B cells. To assess whether IL-10 actually promotes the DNA recombination underlying switching to these isotypes, we examined the effect of IL-10 on the generation of reciprocal products that form DNA circles as by-products of switch recombination. The content of reciprocal products characteristic of mu-gamma recombination was elevated after culture of CD40-activated tonsillar sIgD+ B cells with either IL-4 or IL-10, although high levels of IgG secretion were observed only with IL-10. Unlike IL-4, IL-10 did not induce reciprocal products of mu-epsilon and gamma-epsilon switch recombination. These results demonstrate that IL- 10 promotes both switching to gamma and IgG secretion. The Rockefeller University Press 1996-03-01 /pmc/articles/PMC2192363/ /pubmed/8642297 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
title Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
spellingShingle Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
title_short Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
title_full Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
title_fullStr Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
title_full_unstemmed Interleukin-10 induces immunoglobulin G isotype switch recombination in human CD40-activated naive B lymphocytes
title_sort interleukin-10 induces immunoglobulin g isotype switch recombination in human cd40-activated naive b lymphocytes
description Upon activation, B lymphocytes can change the isotype of the antibody they express by immunoglobulin (Ig) isotype switch recombination. In previous studies on the regulation of human IgG expression, we demonstrated that interleukin 10 (IL-10) could stimulate IgG1 and IgG3 secretion by human CD40-activated naive (sIgD+) tonsillar B cells. To assess whether IL-10 actually promotes the DNA recombination underlying switching to these isotypes, we examined the effect of IL-10 on the generation of reciprocal products that form DNA circles as by-products of switch recombination. The content of reciprocal products characteristic of mu-gamma recombination was elevated after culture of CD40-activated tonsillar sIgD+ B cells with either IL-4 or IL-10, although high levels of IgG secretion were observed only with IL-10. Unlike IL-4, IL-10 did not induce reciprocal products of mu-epsilon and gamma-epsilon switch recombination. These results demonstrate that IL- 10 promotes both switching to gamma and IgG secretion.
publisher The Rockefeller University Press
publishDate 1996
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192363/
_version_ 1611430447259058176

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