gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes

gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes

T cells expressing gamma/delta T cell receptors home to epithelial tissue and may play a role in immunity to infectious agents and foreign antigens. In an effort to understand the role of gamma/delta T cells in directing B cell responses, we investigated the capacity of human gamma/delta T cells to...

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Format: Online
Language:English
Published: The Rockefeller University Press 1995
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191938/
id pubmed-2191938
recordtype oai_dc
spelling pubmed-21919382008-04-16 gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes Articles T cells expressing gamma/delta T cell receptors home to epithelial tissue and may play a role in immunity to infectious agents and foreign antigens. In an effort to understand the role of gamma/delta T cells in directing B cell responses, we investigated the capacity of human gamma/delta T cells to express CD40 ligand (CD40L) and to drive immunoglobulin (Ig) isotype switching in B cells. A multiple step purification procedure resulted in the recovery of highly pure populations of peripheral blood CD4-CD8- gamma/delta T cells. Neither CD40L surface expression nor CD40L mRNA were detected in unstimulated gamma/delta T cells. Stimulation with phorbol ester and ionomycin induced CD40L mRNA and surface CD40L expression by gamma/delta T cells. Both the percentage of CD40L+ cells and the cell surface density of CD40L were significantly lower in gamma/delta T cells compared to unselected T cells. We further demonstrated that in the presence of neutralizing monoclonal antibody to interferon gamma (IFN-gamma), gamma/delta T cells could induce IgE synthesis in B cells, albeit to a lesser extent than unselected T cells. Furthermore, IgE synthesis driven by gamma/delta T cells was inhibited by monoclonal antibody to CD40L. These observations demonstrate that activated gamma/delta T cells express CD40L and can induce isotype switching in B cells. The Rockefeller University Press 1995-03-01 /pmc/articles/PMC2191938/ /pubmed/7869041 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
title gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
spellingShingle gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
title_short gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
title_full gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
title_fullStr gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
title_full_unstemmed gamma/delta T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes
title_sort gamma/delta t lymphocytes express cd40 ligand and induce isotype switching in b lymphocytes
description T cells expressing gamma/delta T cell receptors home to epithelial tissue and may play a role in immunity to infectious agents and foreign antigens. In an effort to understand the role of gamma/delta T cells in directing B cell responses, we investigated the capacity of human gamma/delta T cells to express CD40 ligand (CD40L) and to drive immunoglobulin (Ig) isotype switching in B cells. A multiple step purification procedure resulted in the recovery of highly pure populations of peripheral blood CD4-CD8- gamma/delta T cells. Neither CD40L surface expression nor CD40L mRNA were detected in unstimulated gamma/delta T cells. Stimulation with phorbol ester and ionomycin induced CD40L mRNA and surface CD40L expression by gamma/delta T cells. Both the percentage of CD40L+ cells and the cell surface density of CD40L were significantly lower in gamma/delta T cells compared to unselected T cells. We further demonstrated that in the presence of neutralizing monoclonal antibody to interferon gamma (IFN-gamma), gamma/delta T cells could induce IgE synthesis in B cells, albeit to a lesser extent than unselected T cells. Furthermore, IgE synthesis driven by gamma/delta T cells was inhibited by monoclonal antibody to CD40L. These observations demonstrate that activated gamma/delta T cells express CD40L and can induce isotype switching in B cells.
publisher The Rockefeller University Press
publishDate 1995
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191938/
_version_ 1611430180658610176

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