Role of arginase in killing of schistosomula of schistosoma mansoni
Nonspecific resistance to the multicellular organism Schistosoma mansoni can be induced in mice by several infectious agents. We utilized the observed genetic restriction of such acquired resistance to study the mediators of killing of the larval stage of S. mansoni in vitro. Adherent peritoneal cel...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1980
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185870/ |
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pubmed-21858702008-04-17 Role of arginase in killing of schistosomula of schistosoma mansoni Olds, GR Ellner, JJ Kearse, LA Kazura, JW Mahmoud, AAF Articles Nonspecific resistance to the multicellular organism Schistosoma mansoni can be induced in mice by several infectious agents. We utilized the observed genetic restriction of such acquired resistance to study the mediators of killing of the larval stage of S. mansoni in vitro. Adherent peritoneal cell monolayers from Corynebacterium parvum-treated C57BL/6J but not from C. parvum-treated BALB/cJ mice killed an increased proportion of schistosomula in 24 h. Activated macrophages (Mφ) from both strains exhibited enhanced H(2)0(2) production after incubation with the parasites or phorbol myristate acetate. Thus H(2)0(2) production was not associated with schistosomula killing. Moreover, schistosomula killing was unaffected by catalase or superoxide dismutase. In contrast, activated C57BL/6J (but not BALB/cJ) Mφ released fourfold more arginase into supernates than control Mφ. Schistosomula killing by these Mφ correlated with arginase content of the supernates, was exaggerated in arginine-poor medium, and could be blocked by the addition of arginine. Exogenous bovine arginase added to Fischer's medium without macrophages produced comparable parasite mortality. Our data suggest that arginase is a critical mediator of in vitro killing of this multicellular organism by activated macrophages. The Rockefeller University Press 1980-06-01 /pmc/articles/PMC2185870/ /pubmed/7381366 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Olds, GR Ellner, JJ Kearse, LA Kazura, JW Mahmoud, AAF |
| spellingShingle |
Olds, GR Ellner, JJ Kearse, LA Kazura, JW Mahmoud, AAF Role of arginase in killing of schistosomula of schistosoma mansoni |
| author_facet |
Olds, GR Ellner, JJ Kearse, LA Kazura, JW Mahmoud, AAF |
| author_sort |
Olds, GR |
| title |
Role of arginase in killing of schistosomula of schistosoma mansoni |
| title_short |
Role of arginase in killing of schistosomula of schistosoma mansoni |
| title_full |
Role of arginase in killing of schistosomula of schistosoma mansoni |
| title_fullStr |
Role of arginase in killing of schistosomula of schistosoma mansoni |
| title_full_unstemmed |
Role of arginase in killing of schistosomula of schistosoma mansoni |
| title_sort |
role of arginase in killing of schistosomula of schistosoma mansoni |
| description |
Nonspecific resistance to the multicellular organism Schistosoma mansoni can be induced in mice by several infectious agents. We utilized the observed genetic restriction of such acquired resistance to study the mediators of killing of the larval stage of S. mansoni in vitro. Adherent peritoneal cell monolayers from Corynebacterium parvum-treated C57BL/6J but not from C. parvum-treated BALB/cJ mice killed an increased proportion of schistosomula in 24 h. Activated macrophages (Mφ) from both strains exhibited enhanced H(2)0(2) production after incubation with the parasites or phorbol myristate acetate. Thus H(2)0(2) production was not associated with schistosomula killing. Moreover, schistosomula killing was unaffected by catalase or superoxide dismutase. In contrast, activated C57BL/6J (but not BALB/cJ) Mφ released fourfold more arginase into supernates than control Mφ. Schistosomula killing by these Mφ correlated with arginase content of the supernates, was exaggerated in arginine-poor medium, and could be blocked by the addition of arginine. Exogenous bovine arginase added to Fischer's medium without macrophages produced comparable parasite mortality. Our data suggest that arginase is a critical mediator of in vitro killing of this multicellular organism by activated macrophages. |
| publisher |
The Rockefeller University Press |
| publishDate |
1980 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185870/ |
| _version_ |
1611426887093977088 |