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pubmed-2184107
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| recordtype |
oai_dc
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| spelling |
pubmed-21841072008-04-17 Culture of mononuclear phagocytes on a teflon surface to prevent adherence Articles A method is described for the culture of mononuclear phagocytes in suspension by incubation on a Teflon film to which the cells do not adhere. The characteristics of peritoneal macrophages, bone marrow mononuclear phagocytes, macrophage cell lines, and fibroblasts cultured in this way are similar to those observed after culture on glass or plastic surfaces. Culture of mononuclear phagocytes in Teflon film dishes has three important advantages: the cells can be easily harvested without damage, recovery is almost complete, and the cells are not functionally impaired. Thus, this method makes it possible to use cultured mononuclear phagocytes for many studied that could previously only be done in freshly collected cells. The Rockefeller University Press 1978-01-01 /pmc/articles/PMC2184107/ /pubmed/342666 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
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| repository_type |
Open Access Journal
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| institution_category |
Foreign Institution
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| institution |
US National Center for Biotechnology Information
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| building |
NCBI PubMed
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| collection |
Online Access
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| language |
English
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| format |
Online
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| title |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| spellingShingle |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| title_short |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| title_full |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| title_fullStr |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| title_full_unstemmed |
Culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| title_sort |
culture of mononuclear phagocytes on a teflon surface to prevent adherence
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| description |
A method is described for the culture of mononuclear phagocytes in suspension by incubation on a Teflon film to which the cells do not adhere. The characteristics of peritoneal macrophages, bone marrow mononuclear phagocytes, macrophage cell lines, and fibroblasts cultured in this way are similar to those observed after culture on glass or plastic surfaces. Culture of mononuclear phagocytes in Teflon film dishes has three important advantages: the cells can be easily harvested without damage, recovery is almost complete, and the cells are not functionally impaired. Thus, this method makes it possible to use cultured mononuclear phagocytes for many studied that could previously only be done in freshly collected cells.
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| publisher |
The Rockefeller University Press
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| publishDate |
1978
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| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184107/
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| _version_ |
1611426428804399104
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