Preparative purification of the rat mast cell chymase. Characterization and Interaction with granule components

The rat mast cell granule chymotrypsinlike enzyme was purified to homogeneity from 1 M NaCl solubilized membrane and granule-rich fractions of concentrated rat peritoneal mast cells by a preparative technique utilizing chromatography on Dowex 1, filtration on Sephadex G-75, and affinity chromatograp...

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Bibliographic Details
Main Authors: Yurt, R, Austen, KF
Format: Online
Language:English
Published: The Rockefeller University Press 1977
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180961/