Preparative purification of the rat mast cell chymase. Characterization and Interaction with granule components
The rat mast cell granule chymotrypsinlike enzyme was purified to homogeneity from 1 M NaCl solubilized membrane and granule-rich fractions of concentrated rat peritoneal mast cells by a preparative technique utilizing chromatography on Dowex 1, filtration on Sephadex G-75, and affinity chromatograp...
Main Authors: | , |
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Format: | Online |
Language: | English |
Published: |
The Rockefeller University Press
1977
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180961/ |