The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system

The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system

The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and di...

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Main Authors: Schaeren-Wiemers, Nicole, Bonnet, Annick, Erb, Michael, Erne, Beat, Bartsch, Udo, Kern, Frances, Mantei, Ned, Sherman, Diane, Suter, Ueli
Format: Online
Language:English
Published: The Rockefeller University Press 2004
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172435/
id pubmed-2172435
recordtype oai_dc
spelling pubmed-21724352008-03-05 The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system Schaeren-Wiemers, Nicole Bonnet, Annick Erb, Michael Erne, Beat Bartsch, Udo Kern, Frances Mantei, Ned Sherman, Diane Suter, Ueli Research Articles The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and disorganized transverse bands at the paranode–axon interface in the adult central nervous system. These structural changes were accompanied by a marked reduction of contactin-associated protein/paranodin, neurofascin 155 (NF155), and the potassium channel Kv1.2, whereas nodal clusters of sodium channels were unaltered. Initial formation of paranodal regions appeared normal, but abnormalities became detectable when MAL started to be expressed. Biochemical analysis revealed reduced myelin-associated glycoprotein, myelin basic protein, and NF155 protein levels in myelin and myelin-derived rafts. Our results demonstrate a critical role for MAL in the maintenance of central nervous system paranodes, likely by controlling the trafficking and/or sorting of NF155 and other membrane components in oligodendrocytes. The Rockefeller University Press 2004-08-30 /pmc/articles/PMC2172435/ /pubmed/15337780 http://dx.doi.org/10.1083/jcb.200406092 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Schaeren-Wiemers, Nicole
Bonnet, Annick
Erb, Michael
Erne, Beat
Bartsch, Udo
Kern, Frances
Mantei, Ned
Sherman, Diane
Suter, Ueli
spellingShingle Schaeren-Wiemers, Nicole
Bonnet, Annick
Erb, Michael
Erne, Beat
Bartsch, Udo
Kern, Frances
Mantei, Ned
Sherman, Diane
Suter, Ueli
The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
author_facet Schaeren-Wiemers, Nicole
Bonnet, Annick
Erb, Michael
Erne, Beat
Bartsch, Udo
Kern, Frances
Mantei, Ned
Sherman, Diane
Suter, Ueli
author_sort Schaeren-Wiemers, Nicole
title The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
title_short The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
title_full The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
title_fullStr The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
title_full_unstemmed The raft-associated protein MAL is required for maintenance of proper axon–glia interactions in the central nervous system
title_sort raft-associated protein mal is required for maintenance of proper axon–glia interactions in the central nervous system
description The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and disorganized transverse bands at the paranode–axon interface in the adult central nervous system. These structural changes were accompanied by a marked reduction of contactin-associated protein/paranodin, neurofascin 155 (NF155), and the potassium channel Kv1.2, whereas nodal clusters of sodium channels were unaltered. Initial formation of paranodal regions appeared normal, but abnormalities became detectable when MAL started to be expressed. Biochemical analysis revealed reduced myelin-associated glycoprotein, myelin basic protein, and NF155 protein levels in myelin and myelin-derived rafts. Our results demonstrate a critical role for MAL in the maintenance of central nervous system paranodes, likely by controlling the trafficking and/or sorting of NF155 and other membrane components in oligodendrocytes.
publisher The Rockefeller University Press
publishDate 2004
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172435/
_version_ 1611424927137660928

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