The uniformity of phagosome maturation in macrophages

Many studies of endocytosis and phagocytosis presume that organelles containing a single kind of internalized particle exhibit invariant patterns of protein and phospholipid association as they mature inside cells. To test this presumption, fluorescent protein chimeras were expressed in RAW 264.7 ma...

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Main Authors:	Henry, Rebecca M., Hoppe, Adam D., Joshi, Nikhil, Swanson, Joel A.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2004
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172341/

id	pubmed-2172341
recordtype	oai_dc
spelling	pubmed-21723412008-03-05 The uniformity of phagosome maturation in macrophages Henry, Rebecca M. Hoppe, Adam D. Joshi, Nikhil Swanson, Joel A. Article Many studies of endocytosis and phagocytosis presume that organelles containing a single kind of internalized particle exhibit invariant patterns of protein and phospholipid association as they mature inside cells. To test this presumption, fluorescent protein chimeras were expressed in RAW 264.7 macrophages, and time-lapse ratiometric fluorescence microscopy was used to measure the maturation dynamics of individual phagosomes containing IgG-opsonized erythrocytes. Quantitative analysis revealed consistent patterns of association for YFP chimeras of β-actin, Rab5a, Rab7, and LAMP-1, and no association of YFP chimeras marking endoplasmic reticulum or Golgi. YFP-2xFYVE, recognizing phosphatidylinositol 3-phosphate (PI(3)P), showed two patterns of phagosome labeling. Some phagosomes increased labeling quickly after phagosome closure and then lost the label within 20 min, whereas others labeled more slowly and retained the label for several hours. The two patterns of PI(3)P on otherwise identical phagosomes indicated that organelle maturation does not necessarily follow a single path and that some features of phagosome maturation are integrated over the entire organelle. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172341/ /pubmed/14718518 http://dx.doi.org/10.1083/jcb.200307080 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Henry, Rebecca M. Hoppe, Adam D. Joshi, Nikhil Swanson, Joel A.
spellingShingle	Henry, Rebecca M. Hoppe, Adam D. Joshi, Nikhil Swanson, Joel A. The uniformity of phagosome maturation in macrophages
author_facet	Henry, Rebecca M. Hoppe, Adam D. Joshi, Nikhil Swanson, Joel A.
author_sort	Henry, Rebecca M.
title	The uniformity of phagosome maturation in macrophages
title_short	The uniformity of phagosome maturation in macrophages
title_full	The uniformity of phagosome maturation in macrophages
title_fullStr	The uniformity of phagosome maturation in macrophages
title_full_unstemmed	The uniformity of phagosome maturation in macrophages
title_sort	uniformity of phagosome maturation in macrophages
description	Many studies of endocytosis and phagocytosis presume that organelles containing a single kind of internalized particle exhibit invariant patterns of protein and phospholipid association as they mature inside cells. To test this presumption, fluorescent protein chimeras were expressed in RAW 264.7 macrophages, and time-lapse ratiometric fluorescence microscopy was used to measure the maturation dynamics of individual phagosomes containing IgG-opsonized erythrocytes. Quantitative analysis revealed consistent patterns of association for YFP chimeras of β-actin, Rab5a, Rab7, and LAMP-1, and no association of YFP chimeras marking endoplasmic reticulum or Golgi. YFP-2xFYVE, recognizing phosphatidylinositol 3-phosphate (PI(3)P), showed two patterns of phagosome labeling. Some phagosomes increased labeling quickly after phagosome closure and then lost the label within 20 min, whereas others labeled more slowly and retained the label for several hours. The two patterns of PI(3)P on otherwise identical phagosomes indicated that organelle maturation does not necessarily follow a single path and that some features of phagosome maturation are integrated over the entire organelle.
publisher	The Rockefeller University Press
publishDate	2004
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172341/
_version_	1611424895009292288

The uniformity of phagosome maturation in macrophages

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