PATJ regulates tight junction formation and polarity in mammalian epithelial cells

Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust...

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Main Authors:	Shin, Kunyoo, Straight, Sam, Margolis, Ben
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2005
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171825/

id	pubmed-2171825
recordtype	oai_dc
spelling	pubmed-21718252008-03-05 PATJ regulates tight junction formation and polarity in mammalian epithelial cells Shin, Kunyoo Straight, Sam Margolis, Ben Research Articles Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust and PALS1-associated tight junction protein (PATJ). Although Crumbs and PALS1/Stardust are known to be important for cell polarization, recent studies have suggested that Drosophila PATJ is not essential and its function is unclear. Here, we find that PATJ is targeted to the apical region and tight junctions once cell polarization is initiated. We show using RNAi techniques that reduction in PATJ expression leads to delayed tight junction formation as well as defects in cell polarization. These effects are reversed by reintroduction of PATJ into these RNAi cells. This study provides new functional information on PATJ as a polarity protein and increases our understanding of the Crumbs–PALS1–PATJ complex function in epithelial polarity. The Rockefeller University Press 2005-02-28 /pmc/articles/PMC2171825/ /pubmed/15738264 http://dx.doi.org/10.1083/jcb.200408064 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Shin, Kunyoo Straight, Sam Margolis, Ben
spellingShingle	Shin, Kunyoo Straight, Sam Margolis, Ben PATJ regulates tight junction formation and polarity in mammalian epithelial cells
author_facet	Shin, Kunyoo Straight, Sam Margolis, Ben
author_sort	Shin, Kunyoo
title	PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_short	PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_full	PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_fullStr	PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_full_unstemmed	PATJ regulates tight junction formation and polarity in mammalian epithelial cells
title_sort	patj regulates tight junction formation and polarity in mammalian epithelial cells
description	Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust and PALS1-associated tight junction protein (PATJ). Although Crumbs and PALS1/Stardust are known to be important for cell polarization, recent studies have suggested that Drosophila PATJ is not essential and its function is unclear. Here, we find that PATJ is targeted to the apical region and tight junctions once cell polarization is initiated. We show using RNAi techniques that reduction in PATJ expression leads to delayed tight junction formation as well as defects in cell polarization. These effects are reversed by reintroduction of PATJ into these RNAi cells. This study provides new functional information on PATJ as a polarity protein and increases our understanding of the Crumbs–PALS1–PATJ complex function in epithelial polarity.
publisher	The Rockefeller University Press
publishDate	2005
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171825/
_version_	1611424723763200000

PATJ regulates tight junction formation and polarity in mammalian epithelial cells

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