A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin

Nucleostemin (NS) was identified as a stem cell– and cancer cell–enriched nucleolar protein that controls the proliferation of these cells. Here, we report the mechanism that regulates its dynamic shuttling between the nucleolus and nucleoplasm. The nucleolar residence of nucleostemin involves a tra...

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Main Authors:	Tsai, Robert Y.L., McKay, Ronald D.G.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 2005
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171593/

id	pubmed-2171593
recordtype	oai_dc
spelling	pubmed-21715932008-03-05 A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin Tsai, Robert Y.L. McKay, Ronald D.G. Research Articles Nucleostemin (NS) was identified as a stem cell– and cancer cell–enriched nucleolar protein that controls the proliferation of these cells. Here, we report the mechanism that regulates its dynamic shuttling between the nucleolus and nucleoplasm. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. This cycle is propelled by the GTP binding state of nucleostemin. We propose that a rapid nucleostemin cycle is designed to translate extra- and intra-cellular signals into the amount of nucleostemin in the nucleolus in a bidirectional and fast manner. The Rockefeller University Press 2005-01-17 /pmc/articles/PMC2171593/ /pubmed/15657390 http://dx.doi.org/10.1083/jcb.200409053 Text en Copyright © 2005, Government This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tsai, Robert Y.L. McKay, Ronald D.G.
spellingShingle	Tsai, Robert Y.L. McKay, Ronald D.G. A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
author_facet	Tsai, Robert Y.L. McKay, Ronald D.G.
author_sort	Tsai, Robert Y.L.
title	A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
title_short	A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
title_full	A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
title_fullStr	A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
title_full_unstemmed	A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
title_sort	multistep, gtp-driven mechanism controlling the dynamic cycling of nucleostemin
description	Nucleostemin (NS) was identified as a stem cell– and cancer cell–enriched nucleolar protein that controls the proliferation of these cells. Here, we report the mechanism that regulates its dynamic shuttling between the nucleolus and nucleoplasm. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. This cycle is propelled by the GTP binding state of nucleostemin. We propose that a rapid nucleostemin cycle is designed to translate extra- and intra-cellular signals into the amount of nucleostemin in the nucleolus in a bidirectional and fast manner.
publisher	The Rockefeller University Press
publishDate	2005
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171593/
_version_	1611424657326473216

A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin

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