Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.

In the present study we have used fluorocytometry and immunoprecipitation to characterize the expression of alphav-containing integrins in a panel of eight human breast cancer cell lines and one normal human mammary epithelial line. We show that the classical vitronectin receptor alphavbeta3 is expr...

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Main Authors:	Meyer, T., Marshall, J. F., Hart, I. R.
Format:	Online
Language:	English
Published:	Nature Publishing Group 1998
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2149929/

id	pubmed-2149929
recordtype	oai_dc
spelling	pubmed-21499292009-09-10 Expression of alphav integrins and vitronectin receptor identity in breast cancer cells. Meyer, T. Marshall, J. F. Hart, I. R. Research Article In the present study we have used fluorocytometry and immunoprecipitation to characterize the expression of alphav-containing integrins in a panel of eight human breast cancer cell lines and one normal human mammary epithelial line. We show that the classical vitronectin receptor alphavbeta3 is expressed in only one cell line (MDA-MB-231), whereas alphavbeta5 is expressed on all breast cancer cell lines and alphavbeta1 is expressed on the majority. Using adherence assays to purified ligands in the presence and absence of function-blocking monoclonal antibodies, we have demonstrated that alphavbeta5 mediates adhesion to vitronectin in the majority of these cells. In one cell line, ZR75-1, alphavbeta1 contributes significantly to adhesion to immobilized vitronectin. The formation of focal adhesions containing the alphav and beta1 subunits on vitronectin is also demonstrated by indirect immunofluorescence. Nature Publishing Group 1998-02 /pmc/articles/PMC2149929/ /pubmed/9484807 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Meyer, T. Marshall, J. F. Hart, I. R.
spellingShingle	Meyer, T. Marshall, J. F. Hart, I. R. Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
author_facet	Meyer, T. Marshall, J. F. Hart, I. R.
author_sort	Meyer, T.
title	Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
title_short	Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
title_full	Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
title_fullStr	Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
title_full_unstemmed	Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
title_sort	expression of alphav integrins and vitronectin receptor identity in breast cancer cells.
description	In the present study we have used fluorocytometry and immunoprecipitation to characterize the expression of alphav-containing integrins in a panel of eight human breast cancer cell lines and one normal human mammary epithelial line. We show that the classical vitronectin receptor alphavbeta3 is expressed in only one cell line (MDA-MB-231), whereas alphavbeta5 is expressed on all breast cancer cell lines and alphavbeta1 is expressed on the majority. Using adherence assays to purified ligands in the presence and absence of function-blocking monoclonal antibodies, we have demonstrated that alphavbeta5 mediates adhesion to vitronectin in the majority of these cells. In one cell line, ZR75-1, alphavbeta1 contributes significantly to adhesion to immobilized vitronectin. The formation of focal adhesions containing the alphav and beta1 subunits on vitronectin is also demonstrated by indirect immunofluorescence.
publisher	Nature Publishing Group
publishDate	1998
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2149929/
_version_	1611423664144646144

Expression of alphav integrins and vitronectin receptor identity in breast cancer cells.

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