UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI
1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by...
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
1952
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147362/ |
| id |
pubmed-2147362 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-21473622008-04-23 UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI Daly, Marie M. Allfrey, V. G. Mirsky, A. E. Article 1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by nuclear components of liver, kidney, and pancreas was far more rapid than by those of erythrocytes and sperm. 2. The nuclear components of liver, kidney, and pancreas for which measurements were made were DNA, histone, and residual protein of chromatin. Uptake into DNA was low, into histone higher, and into residual protein much higher still, being comparable with that into mixed cytoplasmic protein. 3. A comparison of the uptake of N15 by the chromosomal components, histone and DNA of liver, pancreas, and kidney showed that chromosomal "activity" varies in different cells and also in the same cell depending upon its over-all activity. The Rockefeller University Press 1952-11-20 /pmc/articles/PMC2147362/ /pubmed/13011275 Text en Copyright © Copyright, 1952, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Daly, Marie M. Allfrey, V. G. Mirsky, A. E. |
| spellingShingle |
Daly, Marie M. Allfrey, V. G. Mirsky, A. E. UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| author_facet |
Daly, Marie M. Allfrey, V. G. Mirsky, A. E. |
| author_sort |
Daly, Marie M. |
| title |
UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| title_short |
UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| title_full |
UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| title_fullStr |
UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| title_full_unstemmed |
UPTAKE OF GLYCINE-N15 BY COMPONENTS OF CELL NUCLEI |
| title_sort |
uptake of glycine-n15 by components of cell nuclei |
| description |
1. The uptake of glycine-N15 by components of cell nuclei was studied. The nuclear components were derived both from tissues with high metabolic rates-mammalian liver, kidney, and pancreas-and from cells with relatively low rates of metabolism-avian erythrocytes and echinoderm sperm. N15 uptake by nuclear components of liver, kidney, and pancreas was far more rapid than by those of erythrocytes and sperm. 2. The nuclear components of liver, kidney, and pancreas for which measurements were made were DNA, histone, and residual protein of chromatin. Uptake into DNA was low, into histone higher, and into residual protein much higher still, being comparable with that into mixed cytoplasmic protein. 3. A comparison of the uptake of N15 by the chromosomal components, histone and DNA of liver, pancreas, and kidney showed that chromosomal "activity" varies in different cells and also in the same cell depending upon its over-all activity. |
| publisher |
The Rockefeller University Press |
| publishDate |
1952 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2147362/ |
| _version_ |
1611423330157461504 |