THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION
Dilute solutions of MnCl2 or MnSO4 accelerate the lytic effect of phage upon susceptible staphylococci. Under the conditions of our experiments the manganese-containing mixtures lysed regularly 0.5 hour sooner than the controls. The effect is shown to be due to a lowering of the lytic threshold, i....
| Main Authors: | , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
1935
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141421/ |
| id |
pubmed-2141421 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-21414212008-04-23 THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION Krueger, A. P. West, N. S. Article Dilute solutions of MnCl2 or MnSO4 accelerate the lytic effect of phage upon susceptible staphylococci. Under the conditions of our experiments the manganese-containing mixtures lysed regularly 0.5 hour sooner than the controls. The effect is shown to be due to a lowering of the lytic threshold, i.e. the quantity of phage/bacterium requisite for lysis; Mn++ reduces the ratio from 54 to about 12. In the presence of Mn++ phage distribution is altered and in growing phage-bacteria mixtures the extracellular phage concentration is increased by manganese to approximately 4 times that occurring in the absence of manganese. There appears to be no enhancement of phage formation nor any affect on the rate of bacterial growth. As would be anticipated, for any given initial phage concentration the end titre after completion of lysis is less in the presence of manganese than in its absence. This is due to the reduced lytic threshold produced by Mn++, there consequently being less phage needed to bring about lytic destruction of the bacteria. The Rockefeller University Press 1935-09-20 /pmc/articles/PMC2141421/ /pubmed/19872916 Text en Copyright © Copyright, 1935, by The Rockefeller Institute for Medical Research This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Krueger, A. P. West, N. S. |
| spellingShingle |
Krueger, A. P. West, N. S. THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| author_facet |
Krueger, A. P. West, N. S. |
| author_sort |
Krueger, A. P. |
| title |
THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| title_short |
THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| title_full |
THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| title_fullStr |
THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| title_full_unstemmed |
THE ACCELERATING EFFECT OF MANGANOUS IONS ON PHAGE ACTION |
| title_sort |
accelerating effect of manganous ions on phage action |
| description |
Dilute solutions of MnCl2 or MnSO4 accelerate the lytic effect of phage upon susceptible staphylococci. Under the conditions of our experiments the manganese-containing mixtures lysed regularly 0.5 hour sooner than the controls. The effect is shown to be due to a lowering of the lytic threshold, i.e. the quantity of phage/bacterium requisite for lysis; Mn++ reduces the ratio from 54 to about 12. In the presence of Mn++ phage distribution is altered and in growing phage-bacteria mixtures the extracellular phage concentration is increased by manganese to approximately 4 times that occurring in the absence of manganese. There appears to be no enhancement of phage formation nor any affect on the rate of bacterial growth. As would be anticipated, for any given initial phage concentration the end titre after completion of lysis is less in the presence of manganese than in its absence. This is due to the reduced lytic threshold produced by Mn++, there consequently being less phage needed to bring about lytic destruction of the bacteria. |
| publisher |
The Rockefeller University Press |
| publishDate |
1935 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141421/ |
| _version_ |
1611422796071567360 |