EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES

The activities of specific transport systems were determined before and after large portions of the surface membrane had been interiorized by phagocytosis of inert particles. In five separate transport systems in rabbit polymorphonuclear leukocytes (adenosine and two adenine transport systems) and...

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Main Authors:	Tsan, Min-Fu, Berlin, Richard D.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1971
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138989/

id	pubmed-2138989
recordtype	oai_dc
spelling	pubmed-21389892008-04-17 EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES Tsan, Min-Fu Berlin, Richard D. Article The activities of specific transport systems were determined before and after large portions of the surface membrane had been interiorized by phagocytosis of inert particles. In five separate transport systems in rabbit polymorphonuclear leukocytes (adenosine and two adenine transport systems) and alveolar macrophages (adenosine and lysine transport systems), the rate of transport was unaffected even after an estimated 35–50% of the membrane had been internalized. Studies of the kinetics of lysine and adenosine transport, exchange diffusion of lysine transport in alveolar macrophages, and the specificities of adenine transport in polymorphonuclear leukocytes indicate that the nature of the membrane transport systems is not altered by phagocytosis. Therefore the constancy of transport indicates that the number of carriers remains the same before and after phagocytosis. It was also shown that this constancy of transport did not depend on the introduction into the surface of new transport sites during phagocytosis. Therefore transport sites are preserved on the surface during the internalization of membrane which accompanies phagocytosis. The results are best explained by the concept that the membrane is mosaic in character with geographically separate transport and phagocytic sites. The Rockefeller University Press 1971-10-01 /pmc/articles/PMC2138989/ /pubmed/5098055 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tsan, Min-Fu Berlin, Richard D.
spellingShingle	Tsan, Min-Fu Berlin, Richard D. EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
author_facet	Tsan, Min-Fu Berlin, Richard D.
author_sort	Tsan, Min-Fu
title	EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
title_short	EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
title_full	EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
title_fullStr	EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
title_full_unstemmed	EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES
title_sort	effect of phagocytosis on membrane transport of nonelectrolytes
description	The activities of specific transport systems were determined before and after large portions of the surface membrane had been interiorized by phagocytosis of inert particles. In five separate transport systems in rabbit polymorphonuclear leukocytes (adenosine and two adenine transport systems) and alveolar macrophages (adenosine and lysine transport systems), the rate of transport was unaffected even after an estimated 35–50% of the membrane had been internalized. Studies of the kinetics of lysine and adenosine transport, exchange diffusion of lysine transport in alveolar macrophages, and the specificities of adenine transport in polymorphonuclear leukocytes indicate that the nature of the membrane transport systems is not altered by phagocytosis. Therefore the constancy of transport indicates that the number of carriers remains the same before and after phagocytosis. It was also shown that this constancy of transport did not depend on the introduction into the surface of new transport sites during phagocytosis. Therefore transport sites are preserved on the surface during the internalization of membrane which accompanies phagocytosis. The results are best explained by the concept that the membrane is mosaic in character with geographically separate transport and phagocytic sites.
publisher	The Rockefeller University Press
publishDate	1971
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138989/
_version_	1611421476845518848

EFFECT OF PHAGOCYTOSIS ON MEMBRANE TRANSPORT OF NONELECTROLYTES

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