Regulation of Actin Polymerization in Cell-free Systems by GTPγS and Cdc42

We have established a cell-free system to investigate pathways that regulate actin polymerization. Addition of GTPγS to lysates of polymorphonuclear leukocytes (PMNs) or Dictyostelium discoideum amoeba induced formation of filamentous actin. The GTPγS appeared to act via a small G-protein, since it...

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Bibliographic Details
Main Authors: Zigmond, Sally H., Joyce, Michael, Borleis, Jane, Bokoch, Gary M., Devreotes, Peter N.
Format: Online
Language:English
Published: The Rockefeller University Press 1997
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138194/