STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS

Efforts were made to elucidate the nature of the resistance to vesicular stomatitis virus (VSV) observed in MCN cultures persistently infected with Newcastle disease, mumps, or 6-6 viruses (MCNNDV, MCNMps and MCN6-6, respectively). Cells derived from persistently infected cultures adsorbed VSV to t...

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Main Authors:	Bergs, Victor V., Henle, Gertrude, Deinhardt, Friedrich, Henle, Werner
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1958
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136894/

id	pubmed-2136894
recordtype	oai_dc
spelling	pubmed-21368942008-04-17 STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS Bergs, Victor V. Henle, Gertrude Deinhardt, Friedrich Henle, Werner Article Efforts were made to elucidate the nature of the resistance to vesicular stomatitis virus (VSV) observed in MCN cultures persistently infected with Newcastle disease, mumps, or 6-6 viruses (MCNNDV, MCNMps and MCN6-6, respectively). Cells derived from persistently infected cultures adsorbed VSV to the same extent as their uninfected counterparts. Only a fraction of the adsorbed virus could be recovered from the cells indicating that it enters into an eclipse in all of the cell types. While propagation of VSV in MCN cells is largely inhibited at low pH levels, the resistance of persistently infected cultures could not be ascribed to their increased lactic acid formation. Resistance was not absolute in that a few cells in persistently infected cultures apparently supported VSV reproduction. Furthermore resistance of the cultures was found to be transitory in that the VSV infection gradually gained the upper hand after 2 to 4 weeks of incubation. Addition of ultraviolet-inactivated NDV to MCN cultures induced resistance to VSV as long as the equivalent of at least one ID50 (for chick embryos) of inactivated virus was provided per cell. Establishment of resistance required some time and its duration depended upon whether or not the free inactivated NDV was removed or neutralized after given adsorption periods. The transitory nature of resistance in persistently infected cultures, or in MCN cells following adsorption of inactivated NDV, is most likely explained by the fact that the cells continue to divide and that the daughter cells are, at least in part, susceptible to VSV. The results are compatible with the conclusion that the resistance observed represents another example of interference between 2 viruses. The Rockefeller University Press 1958-09-30 /pmc/articles/PMC2136894/ /pubmed/13575684 Text en Copyright © Copyright, 1958, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bergs, Victor V. Henle, Gertrude Deinhardt, Friedrich Henle, Werner
spellingShingle	Bergs, Victor V. Henle, Gertrude Deinhardt, Friedrich Henle, Werner STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
author_facet	Bergs, Victor V. Henle, Gertrude Deinhardt, Friedrich Henle, Werner
author_sort	Bergs, Victor V.
title	STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
title_short	STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
title_full	STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
title_fullStr	STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
title_full_unstemmed	STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS
title_sort	studies on persistent infections of tissue cultures : ii. nature of the resistance to vesicular stomatitis virus
description	Efforts were made to elucidate the nature of the resistance to vesicular stomatitis virus (VSV) observed in MCN cultures persistently infected with Newcastle disease, mumps, or 6-6 viruses (MCNNDV, MCNMps and MCN6-6, respectively). Cells derived from persistently infected cultures adsorbed VSV to the same extent as their uninfected counterparts. Only a fraction of the adsorbed virus could be recovered from the cells indicating that it enters into an eclipse in all of the cell types. While propagation of VSV in MCN cells is largely inhibited at low pH levels, the resistance of persistently infected cultures could not be ascribed to their increased lactic acid formation. Resistance was not absolute in that a few cells in persistently infected cultures apparently supported VSV reproduction. Furthermore resistance of the cultures was found to be transitory in that the VSV infection gradually gained the upper hand after 2 to 4 weeks of incubation. Addition of ultraviolet-inactivated NDV to MCN cultures induced resistance to VSV as long as the equivalent of at least one ID50 (for chick embryos) of inactivated virus was provided per cell. Establishment of resistance required some time and its duration depended upon whether or not the free inactivated NDV was removed or neutralized after given adsorption periods. The transitory nature of resistance in persistently infected cultures, or in MCN cells following adsorption of inactivated NDV, is most likely explained by the fact that the cells continue to divide and that the daughter cells are, at least in part, susceptible to VSV. The results are compatible with the conclusion that the resistance observed represents another example of interference between 2 viruses.
publisher	The Rockefeller University Press
publishDate	1958
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136894/
_version_	1611420300218466304

STUDIES ON PERSISTENT INFECTIONS OF TISSUE CULTURES : II. NATURE OF THE RESISTANCE TO VESICULAR STOMATITIS VIRUS

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