STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE

The erythrocytic stages of Plasmodium lophurae were freed from their host red cells by specific hemolysis directly into a favorable medium containing an extract of duck erythrocytes. Extracellular survival and development of the parasite in vitro occurred in culture media consisting essentially of...

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Main Author:	Trager, William
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1950
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136043/

id	pubmed-2136043
recordtype	oai_dc
spelling	pubmed-21360432008-04-17 STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE Trager, William Article The erythrocytic stages of Plasmodium lophurae were freed from their host red cells by specific hemolysis directly into a favorable medium containing an extract of duck erythrocytes. Extracellular survival and development of the parasite in vitro occurred in culture media consisting essentially of a very concentrated extract of duck red cells prepared in a special nutrient solution. Omission or dilution of the red cell extract resulted in rapid degeneration of the parasites. Their survival and development were favored by the presence in the erythrocyte extract of gelatin, yeast adenylic acid, and cozymase, and especially by the further addition of adenosinetriphosphate and sodium pyruvate. Under the best conditions yet tested, all the free parasites continued their development extracellularly during the first two days of cultivation. Merozoites formed by the extracellular segmentation of the free parasites originally present developed further into trophozoites. On the third day a majority of the free parasites were still of normal appearance, but by the fourth day more were degenerate, and very few normal parasites remained on the fifth day. The Rockefeller University Press 1950-09-30 /pmc/articles/PMC2136043/ /pubmed/14778916 Text en Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Trager, William
spellingShingle	Trager, William STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
author_facet	Trager, William
author_sort	Trager, William
title	STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
title_short	STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
title_full	STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
title_fullStr	STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
title_full_unstemmed	STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE
title_sort	studies on the extracellular cultivation of an intracellular parasite (avian malaria) : i. development of the organisms in erythrocyte extracts, and the favoring effect of adenosinetriphosphate
description	The erythrocytic stages of Plasmodium lophurae were freed from their host red cells by specific hemolysis directly into a favorable medium containing an extract of duck erythrocytes. Extracellular survival and development of the parasite in vitro occurred in culture media consisting essentially of a very concentrated extract of duck red cells prepared in a special nutrient solution. Omission or dilution of the red cell extract resulted in rapid degeneration of the parasites. Their survival and development were favored by the presence in the erythrocyte extract of gelatin, yeast adenylic acid, and cozymase, and especially by the further addition of adenosinetriphosphate and sodium pyruvate. Under the best conditions yet tested, all the free parasites continued their development extracellularly during the first two days of cultivation. Merozoites formed by the extracellular segmentation of the free parasites originally present developed further into trophozoites. On the third day a majority of the free parasites were still of normal appearance, but by the fourth day more were degenerate, and very few normal parasites remained on the fifth day.
publisher	The Rockefeller University Press
publishDate	1950
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2136043/
_version_	1611419820379602944

STUDIES ON THE EXTRACELLULAR CULTIVATION OF AN INTRACELLULAR PARASITE (AVIAN MALARIA) : I. DEVELOPMENT OF THE ORGANISMS IN ERYTHROCYTE EXTRACTS, AND THE FAVORING EFFECT OF ADENOSINETRIPHOSPHATE

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