IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY

1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg...

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Main Authors:	Kabat, Elvin A., Bendich, Aaron, Bezer, Ada E.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1946
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135588/

id	pubmed-2135588
recordtype	oai_dc
spelling	pubmed-21355882008-04-18 IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY Kabat, Elvin A. Bendich, Aaron Bezer, Ada E. Article 1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg. antibody nitrogen will result in an error of ±12 per cent in the estimation of the amount of A substance. 3. The blood group A substance showed no significant loss of activity at 37°C. after 48 hours at pH 1.07 to 10.7 or after 2 hours at 100°C. over a pH range from 2.97 to 7.58. Exposure at 100°C. at pH 1.03 or at 9.03 or higher resulted in loss of activity. Parallel results were obtained by the hemagglutination inhibition and quantitative precipitin methods. 4. The solubility of specific precipitates of the blood group A substance from hog stomach and its homologous antibody formed in man was found to be about 1.6 µg. antibody N/ml. 5. A comparison is given of the chemical properties and activity of blood group A substances obtained by several procedures from pools of hog stomachs. The Rockefeller University Press 1946-05-31 /pmc/articles/PMC2135588/ /pubmed/19871544 Text en Copyright © Copyright, 1946, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kabat, Elvin A. Bendich, Aaron Bezer, Ada E.
spellingShingle	Kabat, Elvin A. Bendich, Aaron Bezer, Ada E. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
author_facet	Kabat, Elvin A. Bendich, Aaron Bezer, Ada E.
author_sort	Kabat, Elvin A.
title	IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_short	IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_full	IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_fullStr	IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_full_unstemmed	IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY
title_sort	immunochemical studies on blood groups : ii. properties of the blood group a substance from pools of hoo stomachs and of specific precipitates composed of "a" substance and homologous human antibody
description	1. The microquantitative precipitin method can be used to compare the relative activity of different preparations of the blood group A substance from hog stomachs and to study the effect of chemical treatment upon its stability. 2. With samples of about 25 µg. antibody nitrogen, an error of ±1.7 µg. antibody nitrogen will result in an error of ±12 per cent in the estimation of the amount of A substance. 3. The blood group A substance showed no significant loss of activity at 37°C. after 48 hours at pH 1.07 to 10.7 or after 2 hours at 100°C. over a pH range from 2.97 to 7.58. Exposure at 100°C. at pH 1.03 or at 9.03 or higher resulted in loss of activity. Parallel results were obtained by the hemagglutination inhibition and quantitative precipitin methods. 4. The solubility of specific precipitates of the blood group A substance from hog stomach and its homologous antibody formed in man was found to be about 1.6 µg. antibody N/ml. 5. A comparison is given of the chemical properties and activity of blood group A substances obtained by several procedures from pools of hog stomachs.
publisher	The Rockefeller University Press
publishDate	1946
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135588/
_version_	1611419555662397440

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : II. PROPERTIES OF THE BLOOD GROUP A SUBSTANCE FROM POOLS OF HOO STOMACHS AND OF SPECIFIC PRECIPITATES COMPOSED OF "A" SUBSTANCE AND HOMOLOGOUS HUMAN ANTIBODY

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