Nucleocytoplasmic Shuttling Factors Including Ran and CRM1 Mediate Nuclear Export of NFAT In Vitro

We have developed a permeabilized cell assay to study the nuclear export of the shuttling transcription factor NFAT, which contains a leucine-rich export signal. The assay uses HeLa cells that are stably transfected with NFAT fused to the green fluorescent protein (GFP). Nuclear export of GFP–NFAT i...

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Bibliographic Details
Main Authors: Kehlenbach, Ralph H., Dickmanns, Achim, Gerace, Larry
Format: Online
Language:English
Published: The Rockefeller University Press 1998
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132762/