A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS

Methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, Micrococcus catarrhalis, R pneumococcus, Streptococcus hemolyticus, Staphylococcus aureus. No essential differences between the nucleoproteins and the...

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Main Authors:	Boor, Alden K., Miller, C. Phillip
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1934
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132337/

id	pubmed-2132337
recordtype	oai_dc
spelling	pubmed-21323372008-04-18 A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS Boor, Alden K. Miller, C. Phillip Article Methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, Micrococcus catarrhalis, R pneumococcus, Streptococcus hemolyticus, Staphylococcus aureus. No essential differences between the nucleoproteins and the intact cells of gonococcus and meningococcus were observed in their ability to engender immune substances (precipitins), to induce bacterial allergy in rabbits, or to elicit cutaneous reactions (of the delayed type) in rabbits rendered hypersensitive to these organisms. Measured by their lethal action in mice, the toxicity of gonococcal and meningococcal nucleoproteins was found to be but slightly less than that of the intact cells. It seems probable, therefore, that the toxic action of these organisms is due, chiefly or entirely, to some constituent of the nucleoprotein fraction. Extraction with acetone and ether in the cold did not reduce appreciably the toxicity of these organisms and their nucleoproteins, nor alter their immunological behavior. Cross-precipitin reactions suggested that gonococcal nucleoprotein contains an antigenic factor in common with the non-encapsulated pneumococcus cell, and meningococcal nucleoprotein one in common with the capsular material of Pneumococcus Type III. Tryptic digestion destroys these antigenic factors, but not those responsible for the cross-reactions within the genus Neisseria. The Rockefeller University Press 1934-01-01 /pmc/articles/PMC2132337/ /pubmed/19870231 Text en Copyright © Copyright, 1934, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Boor, Alden K. Miller, C. Phillip
spellingShingle	Boor, Alden K. Miller, C. Phillip A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
author_facet	Boor, Alden K. Miller, C. Phillip
author_sort	Boor, Alden K.
title	A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
title_short	A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
title_full	A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
title_fullStr	A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
title_full_unstemmed	A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS
title_sort	study on bacterial proteins with special consideration of gonococcus and meningococcus
description	Methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, Micrococcus catarrhalis, R pneumococcus, Streptococcus hemolyticus, Staphylococcus aureus. No essential differences between the nucleoproteins and the intact cells of gonococcus and meningococcus were observed in their ability to engender immune substances (precipitins), to induce bacterial allergy in rabbits, or to elicit cutaneous reactions (of the delayed type) in rabbits rendered hypersensitive to these organisms. Measured by their lethal action in mice, the toxicity of gonococcal and meningococcal nucleoproteins was found to be but slightly less than that of the intact cells. It seems probable, therefore, that the toxic action of these organisms is due, chiefly or entirely, to some constituent of the nucleoprotein fraction. Extraction with acetone and ether in the cold did not reduce appreciably the toxicity of these organisms and their nucleoproteins, nor alter their immunological behavior. Cross-precipitin reactions suggested that gonococcal nucleoprotein contains an antigenic factor in common with the non-encapsulated pneumococcus cell, and meningococcal nucleoprotein one in common with the capsular material of Pneumococcus Type III. Tryptic digestion destroys these antigenic factors, but not those responsible for the cross-reactions within the genus Neisseria.
publisher	The Rockefeller University Press
publishDate	1934
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132337/
_version_	1611418285258047488

A STUDY ON BACTERIAL PROTEINS WITH SPECIAL CONSIDERATION OF GONOCOCCUS AND MENINGOCOCCUS

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