Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans

Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans

We have been investigating a set of genes, collectively called mups, that are essential to striated body wall muscle cell positioning in Caenorhabditis elegans. Here we report our detailed characterization of the mup-2 locus, which encodes troponin T (TnT). Mutants for a heat- sensitive allele, call...

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Format: Online
Language:English
Published: The Rockefeller University Press 1996
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120761/
id pubmed-2120761
recordtype oai_dc
spelling pubmed-21207612008-05-01 Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans Articles We have been investigating a set of genes, collectively called mups, that are essential to striated body wall muscle cell positioning in Caenorhabditis elegans. Here we report our detailed characterization of the mup-2 locus, which encodes troponin T (TnT). Mutants for a heat- sensitive allele, called mup-2(e2346ts), and for a putative null, called mup-2(up1), are defective for embryonic body wall muscle cell contraction, sarcomere organization, and cell positioning. Characterizations of the heat-sensitive allele demonstrate that mutants are also defective for regulated muscle contraction in larval and adult body wall muscle, defective for function of the nonstriated oviduct myoepithelial sheath, and defective for epidermal morphogenesis. We cloned the mup-2 locus and its corresponding cDNA. The cDNA encodes a predicted 405-amino acid protein homologous to vertebrate and invertebrate TnT and includes an invertebrate-specific COOH-terminal tail. The mup-2 mutations lie within these cDNA sequences: mup-2(up1) is a termination codon near NH2 terminus (Glu94) and mup-2(e2346ts) is a termination codon in the COOH-terminal invertebrate-specific tail (Trp342). TnT is a muscle contractile protein that, in association with the thin filament proteins tropomyosin, troponin I and troponin C, regulates myosin-actin interaction in response to a rise in intracellular Ca2+. Our findings demonstrate multiple essential functions for TnT and provide a basis to investigate the in vivo functions and protein interactions of TnT in striated and nonstriated muscles. The Rockefeller University Press 1996-03-02 /pmc/articles/PMC2120761/ /pubmed/8601585 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
title Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
spellingShingle Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
title_short Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
title_full Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
title_fullStr Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
title_full_unstemmed Developmental genetic analysis of troponin T mutations in striated and nonstriated muscle cells of Caenorhabditis elegans
title_sort developmental genetic analysis of troponin t mutations in striated and nonstriated muscle cells of caenorhabditis elegans
description We have been investigating a set of genes, collectively called mups, that are essential to striated body wall muscle cell positioning in Caenorhabditis elegans. Here we report our detailed characterization of the mup-2 locus, which encodes troponin T (TnT). Mutants for a heat- sensitive allele, called mup-2(e2346ts), and for a putative null, called mup-2(up1), are defective for embryonic body wall muscle cell contraction, sarcomere organization, and cell positioning. Characterizations of the heat-sensitive allele demonstrate that mutants are also defective for regulated muscle contraction in larval and adult body wall muscle, defective for function of the nonstriated oviduct myoepithelial sheath, and defective for epidermal morphogenesis. We cloned the mup-2 locus and its corresponding cDNA. The cDNA encodes a predicted 405-amino acid protein homologous to vertebrate and invertebrate TnT and includes an invertebrate-specific COOH-terminal tail. The mup-2 mutations lie within these cDNA sequences: mup-2(up1) is a termination codon near NH2 terminus (Glu94) and mup-2(e2346ts) is a termination codon in the COOH-terminal invertebrate-specific tail (Trp342). TnT is a muscle contractile protein that, in association with the thin filament proteins tropomyosin, troponin I and troponin C, regulates myosin-actin interaction in response to a rise in intracellular Ca2+. Our findings demonstrate multiple essential functions for TnT and provide a basis to investigate the in vivo functions and protein interactions of TnT in striated and nonstriated muscles.
publisher The Rockefeller University Press
publishDate 1996
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2120761/
_version_ 1611416129435074560

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