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pubmed-2119439
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| recordtype |
oai_dc
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| spelling |
pubmed-21194392008-04-16 Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6 Articles Very late antigens VLA-1, VLA-2, VLA-3, and VLA-6, belonging to the beta 1 subfamily of integrins, have been identified as receptors for different binding domains of laminin (LM). We have detected VLA-6, but not VLA-1 and VLA-2 on a subset (50-70%) of fresh peripheral blood CD3- , CD16+, CD56+ human natural killer (NK) cells by immunofluorimetric and biochemical analysis. Binding assays performed on LM-coated plates showed that 10-15% of NK cells spontaneously adhere to LM, and this adhesion is mediated by VLA-6. Activation of NK cells through CD16 triggering or by phorbol ester results in a rapid increase of adhesion to LM, which is still mediated by VLA-6. The enhanced adhesiveness is not associated with changes in beta 1 LM receptor expression, while it correlates with changes in the phosphorylation status of alpha 6 subunit. The expression of VLA-6 on NK cells and the modulation of its avidity by activating stimuli may be relevant for NK cell migration and tissue location during inflammation or immune response. The Rockefeller University Press 1992-11-01 /pmc/articles/PMC2119439/ /pubmed/1402670 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
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| repository_type |
Open Access Journal
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| institution_category |
Foreign Institution
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| institution |
US National Center for Biotechnology Information
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| building |
NCBI PubMed
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| collection |
Online Access
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| language |
English
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| format |
Online
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| title |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| spellingShingle |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| title_short |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| title_full |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| title_fullStr |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| title_full_unstemmed |
Triggering through CD16 or phorbol esters enhances adhesion of NK cells to laminin via very late antigen 6
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| title_sort |
triggering through cd16 or phorbol esters enhances adhesion of nk cells to laminin via very late antigen 6
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| description |
Very late antigens VLA-1, VLA-2, VLA-3, and VLA-6, belonging to the beta 1 subfamily of integrins, have been identified as receptors for different binding domains of laminin (LM). We have detected VLA-6, but not VLA-1 and VLA-2 on a subset (50-70%) of fresh peripheral blood CD3- , CD16+, CD56+ human natural killer (NK) cells by immunofluorimetric and biochemical analysis. Binding assays performed on LM-coated plates showed that 10-15% of NK cells spontaneously adhere to LM, and this adhesion is mediated by VLA-6. Activation of NK cells through CD16 triggering or by phorbol ester results in a rapid increase of adhesion to LM, which is still mediated by VLA-6. The enhanced adhesiveness is not associated with changes in beta 1 LM receptor expression, while it correlates with changes in the phosphorylation status of alpha 6 subunit. The expression of VLA-6 on NK cells and the modulation of its avidity by activating stimuli may be relevant for NK cell migration and tissue location during inflammation or immune response.
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| publisher |
The Rockefeller University Press
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| publishDate |
1992
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| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119439/
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| _version_ |
1611415351976787968
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