In vitro reconstitution of intercompartmental protein transport to the yeast vacuole
Toward a detailed understanding of protein sorting in the late secretory pathway, we have reconstituted intercompartmental transfer and proteolytic maturation of a yeast vacuolar protease, carboxypeptidase Y (CPY). This in vitro reconstitution uses permeabilized yeast spheroplasts that are first rad...
| Format: | Online |
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| Language: | English |
| Published: |
The Rockefeller University Press
1990
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2116412/ |