Acanthamoeba actin and profilin can be cross-linked between glutamic acid 364 of actin and lysine 115 of profilin
Acanthamoeba profilin was cross-linked to actin via a zero-length isopeptide bond using carbodiimide. The covalently linked 1:1 complex was purified and treated with cyanogen bromide. This cleaves actin into small cyanogen bromide (CNBr) peptides and leaves the profilin intact owing to its lack of m...
| Format: | Online |
|---|---|
| Language: | English |
| Published: |
The Rockefeller University Press
1989
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115722/ |