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pubmed-2115113
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| recordtype |
oai_dc
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pubmed-21151132008-05-01 Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata Articles We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells on substrata composed of intact fibronectin or a fibronectin fragment containing the primary cell-binding domain. However, in contrast to wild-type cells, the glycosaminoglycan- deficient cells did not attach to substrate composed of a heparin- binding fibronectin fragment. Furthermore, the wild-type but not the glycosaminoglycan-deficient cells formed F-actin-containing stress fibers and focal adhesions on substrata composed of intact fibronectin. We propose, therefore, that cell surface proteoglycan(s) participate in the transmembrane linking of intracellular cytoskeletal components to extracellular matrix components which occurs in focal adhesions. The Rockefeller University Press 1988-03-01 /pmc/articles/PMC2115113/ /pubmed/3346331 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
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| repository_type |
Open Access Journal
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| institution_category |
Foreign Institution
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| institution |
US National Center for Biotechnology Information
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| building |
NCBI PubMed
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| collection |
Online Access
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| language |
English
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| format |
Online
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| title |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| spellingShingle |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| title_short |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| title_full |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| title_fullStr |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| title_full_unstemmed |
Adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| title_sort |
adhesion of glycosaminoglycan-deficient chinese hamster ovary cell mutants to fibronectin substrata
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| description |
We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells on substrata composed of intact fibronectin or a fibronectin fragment containing the primary cell-binding domain. However, in contrast to wild-type cells, the glycosaminoglycan- deficient cells did not attach to substrate composed of a heparin- binding fibronectin fragment. Furthermore, the wild-type but not the glycosaminoglycan-deficient cells formed F-actin-containing stress fibers and focal adhesions on substrata composed of intact fibronectin. We propose, therefore, that cell surface proteoglycan(s) participate in the transmembrane linking of intracellular cytoskeletal components to extracellular matrix components which occurs in focal adhesions.
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| publisher |
The Rockefeller University Press
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| publishDate |
1988
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| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115113/
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| _version_ |
1611413628318121984
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