Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines

Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines

Glucocorticoid hormones can regulate the posttranslational maturation of mouse mammary tumor virus (MTV) precursor polyproteins in M1.54, a stably infected rat hepatoma cell line. We have used complement- mediated cytolysis to recover variants of M1.54 that fail to express MTV cell surface glycoprot...

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Format: Online
Language:English
Published: The Rockefeller University Press 1986
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114581/
id pubmed-2114581
recordtype oai_dc
spelling pubmed-21145812008-05-01 Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines Articles Glucocorticoid hormones can regulate the posttranslational maturation of mouse mammary tumor virus (MTV) precursor polyproteins in M1.54, a stably infected rat hepatoma cell line. We have used complement- mediated cytolysis to recover variants of M1.54 that fail to express MTV cell surface glycoproteins in a hormone-regulated manner (Firestone, G.L., and K.R. Yamamoto, 1983, Mol. Cell. Biol., 3:149- 160). One such clonal isolate, CR4, is similar to wild-type with respect to synthesis of MTV mRNAs, production of the MTV glycoprotein precursor (gPr74env) and a glycosylated maturation product (gp51), and hormone-induced processing of two MTV phosphoproteins. In contrast, three viral cell surface glycoproteins (gp78, gp70, and gp32) and one extracellular species (gp70s), which derive from gPr74env in glucocorticoid-treated wild-type cells, fail to appear in CR4. CR4 showed no apparent alterations in proliferation rate, cell shape, or expression of total functional mRNA and bulk glycoproteins. We conclude that the genetic lesion in CR4 defines a highly selective hormone- regulated glycoprotein maturation pathway that alters the fate of a restricted subset of precursor species. The Rockefeller University Press 1986-12-01 /pmc/articles/PMC2114581/ /pubmed/3023398 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
title Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
spellingShingle Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
title_short Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
title_full Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
title_fullStr Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
title_full_unstemmed Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
title_sort glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines
description Glucocorticoid hormones can regulate the posttranslational maturation of mouse mammary tumor virus (MTV) precursor polyproteins in M1.54, a stably infected rat hepatoma cell line. We have used complement- mediated cytolysis to recover variants of M1.54 that fail to express MTV cell surface glycoproteins in a hormone-regulated manner (Firestone, G.L., and K.R. Yamamoto, 1983, Mol. Cell. Biol., 3:149- 160). One such clonal isolate, CR4, is similar to wild-type with respect to synthesis of MTV mRNAs, production of the MTV glycoprotein precursor (gPr74env) and a glycosylated maturation product (gp51), and hormone-induced processing of two MTV phosphoproteins. In contrast, three viral cell surface glycoproteins (gp78, gp70, and gp32) and one extracellular species (gp70s), which derive from gPr74env in glucocorticoid-treated wild-type cells, fail to appear in CR4. CR4 showed no apparent alterations in proliferation rate, cell shape, or expression of total functional mRNA and bulk glycoproteins. We conclude that the genetic lesion in CR4 defines a highly selective hormone- regulated glycoprotein maturation pathway that alters the fate of a restricted subset of precursor species.
publisher The Rockefeller University Press
publishDate 1986
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114581/
_version_ 1611413310119346176

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