Identification by monoclonal antibodies and characterization of human platelet caldesmon
Actin-based gels were prepared from clarified high-salt extracts of human platelets by dialysis against physiological salt buffers. The gel was partially solubilized with 0.3 M KCl. Mice were immunized with the 0.3 M KCl extract of the actin gel, and hybridomas were produced by fusion of spleen cell...
Format: | Online |
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Language: | English |
Published: |
The Rockefeller University Press
1986
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114224/ |