Identification by monoclonal antibodies and characterization of human platelet caldesmon

Actin-based gels were prepared from clarified high-salt extracts of human platelets by dialysis against physiological salt buffers. The gel was partially solubilized with 0.3 M KCl. Mice were immunized with the 0.3 M KCl extract of the actin gel, and hybridomas were produced by fusion of spleen cell...

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Bibliographic Details
Format: Online
Language:English
Published: The Rockefeller University Press 1986
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114224/