End-to-end annealing of microtubules in vitro

Mixtures of pre-formed microtubules, polymerized from chicken erythrocyte and brain tubulin, rapidly anneal end-to-end in vitro in standard microtubule assembly buffer. The erythrocyte tubulin segments in annealed heteropolymers can be distinguished by an immunoelectron microscopic assay that uses a...

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Bibliographic Details
Format: Online
Language:English
Published: The Rockefeller University Press 1986
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2114097/