Transport and topology of galactosyltransferase in endomembranes of HeLa cells

HeLa cell membranes were studied for the distribution and orientation of the Golgi marker enzyme uridine diphosphate-galactose:beta-D-N- acetylglucosamine beta, 1-4 transferase (GT). Short pulse labeling in the presence of [35S]methionine resulted in two precursor species (Mr = 44,000 and 47,000), p...

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Bibliographic Details
Format: Online
Language:English
Published: The Rockefeller University Press 1983
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112579/