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pubmed-2112435
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| recordtype |
oai_dc
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| spelling |
pubmed-21124352008-05-01 Reversible pinocytosis in polymorphonuclear leukocytes Articles Since pinocytosis has only been recently recognized in polymorphonuclear leukocytes (PMNs), little is known about the fate of pinosomes. Here we report that pinosomes can fuse with the cytoplasmic granules of PMNs. We also find that at least for a short period of time after formation, pinosomes can fuse with the plasma membrane and release their contents to the outside. We present a morphological description and biochemical data on the kinetic parameters of a steady state pool of reversible pinosomes in PMNs. In addition, we have developed conditions under which pinosomes continue to form and fuse with the plasma membrane but fail to fuse with the cytoplasmic granules, i.e., only "reversible" pinocytosis occurs. This inhibition of fusion with the granules is not due to an inability of the pinosomes to move from the surface since under these conditions pinosomes labeled with an electron-dense marker can be seen in the cell interior. The Rockefeller University Press 1983-06-01 /pmc/articles/PMC2112435/ /pubmed/6304114 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
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| repository_type |
Open Access Journal
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| institution_category |
Foreign Institution
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| institution |
US National Center for Biotechnology Information
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| building |
NCBI PubMed
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| collection |
Online Access
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| language |
English
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| format |
Online
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| title |
Reversible pinocytosis in polymorphonuclear leukocytes
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| spellingShingle |
Reversible pinocytosis in polymorphonuclear leukocytes
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| title_short |
Reversible pinocytosis in polymorphonuclear leukocytes
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| title_full |
Reversible pinocytosis in polymorphonuclear leukocytes
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| title_fullStr |
Reversible pinocytosis in polymorphonuclear leukocytes
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| title_full_unstemmed |
Reversible pinocytosis in polymorphonuclear leukocytes
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| title_sort |
reversible pinocytosis in polymorphonuclear leukocytes
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| description |
Since pinocytosis has only been recently recognized in polymorphonuclear leukocytes (PMNs), little is known about the fate of pinosomes. Here we report that pinosomes can fuse with the cytoplasmic granules of PMNs. We also find that at least for a short period of time after formation, pinosomes can fuse with the plasma membrane and release their contents to the outside. We present a morphological description and biochemical data on the kinetic parameters of a steady state pool of reversible pinosomes in PMNs. In addition, we have developed conditions under which pinosomes continue to form and fuse with the plasma membrane but fail to fuse with the cytoplasmic granules, i.e., only "reversible" pinocytosis occurs. This inhibition of fusion with the granules is not due to an inability of the pinosomes to move from the surface since under these conditions pinosomes labeled with an electron-dense marker can be seen in the cell interior.
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| publisher |
The Rockefeller University Press
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| publishDate |
1983
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| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112435/
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| _version_ |
1611412068175446016
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