Quick-freeze, deep-etch visualization of the cytoskeleton beneath surface differentiations of intestinal epithelial cells
The cytoskeleton that supports microvilli in intestinal epithelial cells was visualized by the quick-freeze, deep-etch, rotary-replication technique (Heuser and Salpeter. 1979. J. Cell Biol. 82: 150). Before quick freezing, cells were exposed to detergents or broken open physically to clear away the...
| Format: | Online |
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| Language: | English |
| Published: |
The Rockefeller University Press
1981
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111975/ |