Purification of coated vesicles by agarose gel electrophoresis

We have applied agarose gel electrophoresis as a novel step in the purification of clathrin-coated vesicles. Preparations of coated vesicles obtained by sedimentation velocity and isopycnic centrifugation are resolved into two distinct fractions upon electrophoresis. The slower migrating fraction co...

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Bibliographic Details
Format: Online
Language:English
Published: The Rockefeller University Press 1981
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111692/