GROWTH AND LABILITY OF CHAETOPTERUS OOCYTE MITOTIC SPINDLES ISOLATED IN THE PRESENCE OF PORCINE BRAIN TUBULIN

Purified tubulin solutions stabilized and augmented the birefringence (BR) of isolated Chaetopterus spindles. Tubulin was extracted from pig brain tissue in cold PEG buffer (0.1 M piperazine-N-N'-bis[2-ethane sulfonic acid], 1 mM ethylene bis-[oxyethylenenitrilo]tetraacetate, [EGTA], 2.5 mM gu...

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Bibliographic Details
Main Authors: Inoué, Shinya, Borisy, Gary G., Kiehart, Daniel P.
Format: Online
Language:English
Published: The Rockefeller University Press 1974
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109189/