QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE

The distribution of labeled RNA in the optic nerve of the rabbit was studied by quantitative ultrastructural autoradiography after the intraocular injection of [3H]uridine. The highest density of silver grains related to [3H]RNA (27–40 grains/100 µm2) was found in glial cell perikarya; a slightly l...

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Main Authors:	Gambetti, Pierluigi, Autilio-Gambetti, Lucila, Shafer, Brenda, Pfaff, LaDorna
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1973
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109110/

id	pubmed-2109110
recordtype	oai_dc
spelling	pubmed-21091102008-05-01 QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE Gambetti, Pierluigi Autilio-Gambetti, Lucila Shafer, Brenda Pfaff, LaDorna Article The distribution of labeled RNA in the optic nerve of the rabbit was studied by quantitative ultrastructural autoradiography after the intraocular injection of [3H]uridine. The highest density of silver grains related to [3H]RNA (27–40 grains/100 µm2) was found in glial cell perikarya; a slightly lower density was present in the glial nuclei (19–20 grains/100 µm2). Axons (4–5 grains/100 µm2) and myelin (2–3 grains/100 µm2) had the lowest grain densities. 74–83% of all counted grains were located outside the axons. By comparing the grain density distribution over the axon with that expected in the case of an exclusive labeling of the surrounding myelin and glial cell processes, it was concluded that the axons contained a number of grains representing [3H]RNA significantly higher than that expected to scatter from myelin and glial processes. Most of these grains were concentrated at the periphery of the axon and were not related to axonal mitochondria. The Rockefeller University Press 1973-12-01 /pmc/articles/PMC2109110/ /pubmed/4805833 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gambetti, Pierluigi Autilio-Gambetti, Lucila Shafer, Brenda Pfaff, LaDorna
spellingShingle	Gambetti, Pierluigi Autilio-Gambetti, Lucila Shafer, Brenda Pfaff, LaDorna QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
author_facet	Gambetti, Pierluigi Autilio-Gambetti, Lucila Shafer, Brenda Pfaff, LaDorna
author_sort	Gambetti, Pierluigi
title	QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
title_short	QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
title_full	QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
title_fullStr	QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
title_full_unstemmed	QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE
title_sort	quantitative autoradiographic study of labeled rna in rabbit optic nerve after intraocular injection of [3h]uridine
description	The distribution of labeled RNA in the optic nerve of the rabbit was studied by quantitative ultrastructural autoradiography after the intraocular injection of [3H]uridine. The highest density of silver grains related to [3H]RNA (27–40 grains/100 µm2) was found in glial cell perikarya; a slightly lower density was present in the glial nuclei (19–20 grains/100 µm2). Axons (4–5 grains/100 µm2) and myelin (2–3 grains/100 µm2) had the lowest grain densities. 74–83% of all counted grains were located outside the axons. By comparing the grain density distribution over the axon with that expected in the case of an exclusive labeling of the surrounding myelin and glial cell processes, it was concluded that the axons contained a number of grains representing [3H]RNA significantly higher than that expected to scatter from myelin and glial processes. Most of these grains were concentrated at the periphery of the axon and were not related to axonal mitochondria.
publisher	The Rockefeller University Press
publishDate	1973
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109110/
_version_	1611410408985329664

QUANTITATIVE AUTORADIOGRAPHIC STUDY OF LABELED RNA IN RABBIT OPTIC NERVE AFTER INTRAOCULAR INJECTION OF [3H]URIDINE

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