ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS

ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS

Rabbit retinas were incubated in medium containing 500 µCi of [3H]leucine for 3 min, and transferred to medium without isotope for another 7, 17, 37, 57, and 117 min. Retinal pieces were fixed in paraformaldehyde and osmium tetroxide and embedded in Epon. Thin sections were autoradiographed with Il...

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Main Authors: Magalhães, M. M., Coimbra, Antonio
Format: Online
Language:English
Published: The Rockefeller University Press 1973
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108949/
id pubmed-2108949
recordtype oai_dc
spelling pubmed-21089492008-05-01 ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS Magalhães, M. M. Coimbra, Antonio Article Rabbit retinas were incubated in medium containing 500 µCi of [3H]leucine for 3 min, and transferred to medium without isotope for another 7, 17, 37, 57, and 117 min. Retinal pieces were fixed in paraformaldehyde and osmium tetroxide and embedded in Epon. Thin sections were autoradiographed with Ilford L4 emulsion, and a quantitative study of silver grain distribution per Müller cell portion, and per Müller cell organelle, was carried out. Grain density per unit area was high over the middle cell portion at each incubation interval. Silver grains were numerous over background cytoplasm (which comprised free ribosomes) but their percentage was constant at all times and their relative concentration low. Silver grains were numerous and highly concentrated, at pulse incubation, over the rough endoplasmic reticulum (RER) and then decreased sharply, but this decline coincided with an increase over the Golgi complex, peaking at 20 min. Another peak appeared over the cell periphery at 60 min. These findings suggest the simultaneous synthesis of two types of proteins in Müller cells; structural proteins in background cytoplasm and proteins of secretory type in the RER. The Rockefeller University Press 1973-04-01 /pmc/articles/PMC2108949/ /pubmed/4570792 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Magalhães, M. M.
Coimbra, Antonio
spellingShingle Magalhães, M. M.
Coimbra, Antonio
ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
author_facet Magalhães, M. M.
Coimbra, Antonio
author_sort Magalhães, M. M.
title ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
title_short ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
title_full ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
title_fullStr ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
title_full_unstemmed ELECTRON MICROSCOPE AUTORADIOGRAPHIC DETECTION OF SITES OF PROTEIN SYNTHESIS IN THE RABBIT RETINA MÜLLER CELLS
title_sort electron microscope autoradiographic detection of sites of protein synthesis in the rabbit retina müller cells
description Rabbit retinas were incubated in medium containing 500 µCi of [3H]leucine for 3 min, and transferred to medium without isotope for another 7, 17, 37, 57, and 117 min. Retinal pieces were fixed in paraformaldehyde and osmium tetroxide and embedded in Epon. Thin sections were autoradiographed with Ilford L4 emulsion, and a quantitative study of silver grain distribution per Müller cell portion, and per Müller cell organelle, was carried out. Grain density per unit area was high over the middle cell portion at each incubation interval. Silver grains were numerous over background cytoplasm (which comprised free ribosomes) but their percentage was constant at all times and their relative concentration low. Silver grains were numerous and highly concentrated, at pulse incubation, over the rough endoplasmic reticulum (RER) and then decreased sharply, but this decline coincided with an increase over the Golgi complex, peaking at 20 min. Another peak appeared over the cell periphery at 60 min. These findings suggest the simultaneous synthesis of two types of proteins in Müller cells; structural proteins in background cytoplasm and proteins of secretory type in the RER.
publisher The Rockefeller University Press
publishDate 1973
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108949/
_version_ 1611410313549185024

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