FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
0.3–0.5 M MgCl2 was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by secti...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
1972
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108744/ |