THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers
Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1971
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108435/ |
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pubmed-2108435 |
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pubmed-21084352008-05-01 THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers Ross, Russell Article Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell. The Rockefeller University Press 1971-07-01 /pmc/articles/PMC2108435/ /pubmed/4327464 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ross, Russell |
| spellingShingle |
Ross, Russell THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| author_facet |
Ross, Russell |
| author_sort |
Ross, Russell |
| title |
THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| title_short |
THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| title_full |
THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| title_fullStr |
THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| title_full_unstemmed |
THE SMOOTH MUSCLE CELL : II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers |
| title_sort |
smooth muscle cell : ii. growth of smooth muscle in culture and formation of elastic fibers |
| description |
Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell. |
| publisher |
The Rockefeller University Press |
| publishDate |
1971 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108435/ |
| _version_ |
1611410098246123520 |