STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions

STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions

In Chaos chaos streaming, motility, and cytokinesis were inhibited nearly 100% for several hours by 2.5–5 mM sodium adenosine triphosphate (ATP)1 added to culture fluid. All three effects were completely prevented by the addition of equimolar Mg++ or Ca++ ions but not Na+ to the ATP/culture fluid s...

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Main Author: Nachmias, Vivianne T.
Format: Online
Language:English
Published: The Rockefeller University Press 1969
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107603/
id pubmed-2107603
recordtype oai_dc
spelling pubmed-21076032008-05-01 STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions Nachmias, Vivianne T. Article In Chaos chaos streaming, motility, and cytokinesis were inhibited nearly 100% for several hours by 2.5–5 mM sodium adenosine triphosphate (ATP)1 added to culture fluid. All three effects were completely prevented by the addition of equimolar Mg++ or Ca++ ions but not Na+ to the ATP/culture fluid solution. The effects of ATP were not reproduced by EDTA, EGTA, colchicine, or AMP. Sodium pyrophosphate produced about 50% inhibition at 5 mM. Studies with 14C-ATP showed that 5 x 10-5 to 5 x 10-4 mmole of ATP was firmly associated with each milliliter of packed cells after an hour's incubation at 24°C. Labeling studies also showed that prevention of the ATP effects by Mg++ ions was not due to a decrease in the amount of ATP associated with the cells. The Rockefeller University Press 1969-01-01 /pmc/articles/PMC2107603/ /pubmed/4302021 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Nachmias, Vivianne T.
spellingShingle Nachmias, Vivianne T.
STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
author_facet Nachmias, Vivianne T.
author_sort Nachmias, Vivianne T.
title STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
title_short STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
title_full STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
title_fullStr STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
title_full_unstemmed STUDIES ON STREAMING : I. Inhibition of Protoplasmic Streaming and Cytokinesis of Chaos chaos by Adenosine Triphosphate and Reversal by Magnesium and Calcium Ions
title_sort studies on streaming : i. inhibition of protoplasmic streaming and cytokinesis of chaos chaos by adenosine triphosphate and reversal by magnesium and calcium ions
description In Chaos chaos streaming, motility, and cytokinesis were inhibited nearly 100% for several hours by 2.5–5 mM sodium adenosine triphosphate (ATP)1 added to culture fluid. All three effects were completely prevented by the addition of equimolar Mg++ or Ca++ ions but not Na+ to the ATP/culture fluid solution. The effects of ATP were not reproduced by EDTA, EGTA, colchicine, or AMP. Sodium pyrophosphate produced about 50% inhibition at 5 mM. Studies with 14C-ATP showed that 5 x 10-5 to 5 x 10-4 mmole of ATP was firmly associated with each milliliter of packed cells after an hour's incubation at 24°C. Labeling studies also showed that prevention of the ATP effects by Mg++ ions was not due to a decrease in the amount of ATP associated with the cells.
publisher The Rockefeller University Press
publishDate 1969
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107603/
_version_ 1611409604185423872

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