COMBINED CYTOCHEMICAL AND ELECTRON MICROSCOPIC DEMONSTRATION OF β-GLUCURONIDASE ACTIVITY IN RAT LIVER WITH THE USE OF A SIMULTANEOUS COUPLING AZO DYE TECHNIQUE

Rat liver was fixed in formal-cacodylate-sucrose and frozen sections were incubated in a simultaneous-coupling medium containing naphthol AS-BI glucuronide as substrate and hexazonium pararosanilin as the diazo reagent. By light microscopy, the sections demonstrated β-glucuronidase activity as red...

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Bibliographic Details
Main Authors: Hayashi, Masando, Shirahama, Tsuranobu, Cohen, Alan S.
Format: Online
Language:English
Published: The Rockefeller University Press 1968
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107359/