COMBINED CYTOCHEMICAL AND ELECTRON MICROSCOPIC DEMONSTRATION OF β-GLUCURONIDASE ACTIVITY IN RAT LIVER WITH THE USE OF A SIMULTANEOUS COUPLING AZO DYE TECHNIQUE
Rat liver was fixed in formal-cacodylate-sucrose and frozen sections were incubated in a simultaneous-coupling medium containing naphthol AS-BI glucuronide as substrate and hexazonium pararosanilin as the diazo reagent. By light microscopy, the sections demonstrated β-glucuronidase activity as red...
Main Authors: | , , |
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Format: | Online |
Language: | English |
Published: |
The Rockefeller University Press
1968
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107359/ |