FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS

The skin of late embryonic, larval, and young postmetamorphic newts, Taricha torosa, has been examined with particular reference to areas of cellular attachment. Stereo electron microscopic techniques and special staining methods for extracellular materials were utilized in addition to conventional...

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Main Author:	Kelly, Douglas E.
Format:	Online
Language:	English
Published:	The Rockefeller University Press 1966
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106892/

id	pubmed-2106892
recordtype	oai_dc
spelling	pubmed-21068922008-05-01 FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS Kelly, Douglas E. Article The skin of late embryonic, larval, and young postmetamorphic newts, Taricha torosa, has been examined with particular reference to areas of cellular attachment. Stereo electron microscopic techniques and special staining methods for extracellular materials were utilized in addition to conventional avenues of ultrastructural study to investigate the fine architecture of desmosomes, hemidesmosomes, their associated filament systems, and extracellular materials. No evidence has been found that continuity of tonofilaments between adjacent cells exists at desmosomes. Rather, most of the tonofilaments which approach desmosomes (and perhaps also hemidesmosomes) course toward the "attachment plaque" and then loop, either outside the plaque or within it, and return into the main filament tracts of the cell. These facts suggest that the filamentous framework provides intracellular tensile support while adhesion is a product of extracellular materials which accumulate at attachment sites. Evidence is presented that the extracellular material is arranged as pillars or partitions which are continuous with or layered upon the outer unit cell membrane leaflets and adjoined in a discontinuous dense midline of the desmosome. A similar analysis has been made of extracellular materials associated with hemidesmosomes along the basal surface of epidermal cells. An adepidermal globular zone, separating the basal cell boundary from the underlying basal lamina and collagenous lamellae during larval stages, has been interpreted from enzyme and solvent extraction study as a lipid-mucopolysaccharide complex, the function of which remains obscure. These observations are discussed in relation to prevailing theories of cellular adhesion and epidermal differentiation. They appear consistent with the concept that a wide range of adhesive specializations exists in nature, and that the more highly organized of these, such as large desmosomes and hemidesmosomes, serve as strong, highly supported attachment sites, supplemental in function to a more generalized aggregating mechanism. The Rockefeller University Press 1966-01-01 /pmc/articles/PMC2106892/ /pubmed/5901500 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kelly, Douglas E.
spellingShingle	Kelly, Douglas E. FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
author_facet	Kelly, Douglas E.
author_sort	Kelly, Douglas E.
title	FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
title_short	FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
title_full	FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
title_fullStr	FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
title_full_unstemmed	FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS
title_sort	fine structure of desmosomes, hemidesmosomes, and an adepidermal globular layer in developing newt epidermis
description	The skin of late embryonic, larval, and young postmetamorphic newts, Taricha torosa, has been examined with particular reference to areas of cellular attachment. Stereo electron microscopic techniques and special staining methods for extracellular materials were utilized in addition to conventional avenues of ultrastructural study to investigate the fine architecture of desmosomes, hemidesmosomes, their associated filament systems, and extracellular materials. No evidence has been found that continuity of tonofilaments between adjacent cells exists at desmosomes. Rather, most of the tonofilaments which approach desmosomes (and perhaps also hemidesmosomes) course toward the "attachment plaque" and then loop, either outside the plaque or within it, and return into the main filament tracts of the cell. These facts suggest that the filamentous framework provides intracellular tensile support while adhesion is a product of extracellular materials which accumulate at attachment sites. Evidence is presented that the extracellular material is arranged as pillars or partitions which are continuous with or layered upon the outer unit cell membrane leaflets and adjoined in a discontinuous dense midline of the desmosome. A similar analysis has been made of extracellular materials associated with hemidesmosomes along the basal surface of epidermal cells. An adepidermal globular zone, separating the basal cell boundary from the underlying basal lamina and collagenous lamellae during larval stages, has been interpreted from enzyme and solvent extraction study as a lipid-mucopolysaccharide complex, the function of which remains obscure. These observations are discussed in relation to prevailing theories of cellular adhesion and epidermal differentiation. They appear consistent with the concept that a wide range of adhesive specializations exists in nature, and that the more highly organized of these, such as large desmosomes and hemidesmosomes, serve as strong, highly supported attachment sites, supplemental in function to a more generalized aggregating mechanism.
publisher	The Rockefeller University Press
publishDate	1966
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106892/
_version_	1611409182772166656

FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS

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