ISOLATION OF SKELETAL MUSCLE NUCLEI
A method employing aqueous media for isolation of nuclei from rat skeletal muscle is described. The technique involves (a) mincing and then homogenizing in a 0.32 M sucrose-salt solution with a Potter-Elvehjem type homogenizer using a Delrin (an acetal resin) pestle and a carefully controlled, rela...
| Main Authors: | , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
1965
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106724/ |