ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization
Homogenates of fertilized eggs of the sea urchin Paracentrotus lividus were fractionated by differential centrifugation. In addition, whole eggs were fragmented, on a preparative scale, by centrifugation in sea water-sucrose gradients. The fractions and fragments were subsequently assayed for their...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
1964
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106446/ |
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pubmed-2106446 |
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pubmed-21064462008-05-01 ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization Perlmann, Peter Couffer-Kaltenbach, Jane Article Homogenates of fertilized eggs of the sea urchin Paracentrotus lividus were fractionated by differential centrifugation. In addition, whole eggs were fragmented, on a preparative scale, by centrifugation in sea water-sucrose gradients. The fractions and fragments were subsequently assayed for their content of soluble protein antigens described in an earlier publication. Relative concentrations of antigen present in quantitatively isolated cell fractions were estimated by graded antiserum absorption in combination with agar-diffusion technique. Two of six antigens were found to be associated mainly with the low speed sediments. Treatment of the various sediments with hypotonic medium and results obtained with fragmented eggs suggested that these two antigens and possibly a third were probably located in the yolk granules. The other antigens were more evenly distributed among the low speed sediments and the non-sedimented part of the cytoplasm. Only one of the antigens was consistently associated with the microsomal fraction. The Rockefeller University Press 1964-08-01 /pmc/articles/PMC2106446/ /pubmed/14203382 Text en Copyright © 1964 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Perlmann, Peter Couffer-Kaltenbach, Jane |
| spellingShingle |
Perlmann, Peter Couffer-Kaltenbach, Jane ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| author_facet |
Perlmann, Peter Couffer-Kaltenbach, Jane |
| author_sort |
Perlmann, Peter |
| title |
ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| title_short |
ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| title_full |
ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| title_fullStr |
ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| title_full_unstemmed |
ANTIGENS IN EGGS AND DEVELOPMENTAL STAGES OF THE SEA URCHIN : II. Localization |
| title_sort |
antigens in eggs and developmental stages of the sea urchin : ii. localization |
| description |
Homogenates of fertilized eggs of the sea urchin Paracentrotus lividus were fractionated by differential centrifugation. In addition, whole eggs were fragmented, on a preparative scale, by centrifugation in sea water-sucrose gradients. The fractions and fragments were subsequently assayed for their content of soluble protein antigens described in an earlier publication. Relative concentrations of antigen present in quantitatively isolated cell fractions were estimated by graded antiserum absorption in combination with agar-diffusion technique. Two of six antigens were found to be associated mainly with the low speed sediments. Treatment of the various sediments with hypotonic medium and results obtained with fragmented eggs suggested that these two antigens and possibly a third were probably located in the yolk granules. The other antigens were more evenly distributed among the low speed sediments and the non-sedimented part of the cytoplasm. Only one of the antigens was consistently associated with the microsomal fraction. |
| publisher |
The Rockefeller University Press |
| publishDate |
1964 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106446/ |
| _version_ |
1611408918775332864 |