ISOLATION OF SMOOTH VESICLES AND FREE RIBOSOMES FROM RAT LIVER MICROSOMES
Microsomes, isolated from rat liver homogenate in 0.88 M sucrose, have been fractionated by differential centrifugation. The 2nd microsomal fraction, sedimented between 60 minutes at 105,000 g and 3 hours at 145,000 g, consists mainly of smooth vesicles, free ribosomes, and ferritin. By utilizing t...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
The Rockefeller University Press
1962
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106013/ |