A role for a novel centrosome cycle in asymmetric cell division
Tissue stem cells play a key role in tissue maintenance. Drosophila melanogaster central brain neuroblasts are excellent models for stem cell asymmetric division. Earlier work showed that their mitotic spindle orientation is established before spindle formation. We investigated the mechanism by whic...
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| Format: | Online |
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The Rockefeller University Press
2007
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064101/ |
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pubmed-20641012007-11-29 A role for a novel centrosome cycle in asymmetric cell division Rusan, Nasser M. Peifer, Mark Research Articles Tissue stem cells play a key role in tissue maintenance. Drosophila melanogaster central brain neuroblasts are excellent models for stem cell asymmetric division. Earlier work showed that their mitotic spindle orientation is established before spindle formation. We investigated the mechanism by which this occurs, revealing a novel centrosome cycle. In interphase, the two centrioles separate, but only one is active, retaining pericentriolar material and forming a “dominant centrosome.” This centrosome acts as a microtubule organizing center (MTOC) and remains stationary, forming one pole of the future spindle. The second centriole is inactive and moves to the opposite side of the cell before being activated as a centrosome/MTOC. This is accompanied by asymmetric localization of Polo kinase, a key centrosome regulator. Disruption of centrosomes disrupts the high fidelity of asymmetric division. We propose a two-step mechanism to ensure faithful spindle positioning: the novel centrosome cycle produces a single interphase MTOC, coarsely aligning the spindle, and spindle–cortex interactions refine this alignment. The Rockefeller University Press 2007-04-09 /pmc/articles/PMC2064101/ /pubmed/17403931 http://dx.doi.org/10.1083/jcb.200612140 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Rusan, Nasser M. Peifer, Mark |
| spellingShingle |
Rusan, Nasser M. Peifer, Mark A role for a novel centrosome cycle in asymmetric cell division |
| author_facet |
Rusan, Nasser M. Peifer, Mark |
| author_sort |
Rusan, Nasser M. |
| title |
A role for a novel centrosome cycle in asymmetric cell division |
| title_short |
A role for a novel centrosome cycle in asymmetric cell division |
| title_full |
A role for a novel centrosome cycle in asymmetric cell division |
| title_fullStr |
A role for a novel centrosome cycle in asymmetric cell division |
| title_full_unstemmed |
A role for a novel centrosome cycle in asymmetric cell division |
| title_sort |
role for a novel centrosome cycle in asymmetric cell division |
| description |
Tissue stem cells play a key role in tissue maintenance. Drosophila melanogaster central brain neuroblasts are excellent models for stem cell asymmetric division. Earlier work showed that their mitotic spindle orientation is established before spindle formation. We investigated the mechanism by which this occurs, revealing a novel centrosome cycle. In interphase, the two centrioles separate, but only one is active, retaining pericentriolar material and forming a “dominant centrosome.” This centrosome acts as a microtubule organizing center (MTOC) and remains stationary, forming one pole of the future spindle. The second centriole is inactive and moves to the opposite side of the cell before being activated as a centrosome/MTOC. This is accompanied by asymmetric localization of Polo kinase, a key centrosome regulator. Disruption of centrosomes disrupts the high fidelity of asymmetric division. We propose a two-step mechanism to ensure faithful spindle positioning: the novel centrosome cycle produces a single interphase MTOC, coarsely aligning the spindle, and spindle–cortex interactions refine this alignment. |
| publisher |
The Rockefeller University Press |
| publishDate |
2007 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064101/ |
| _version_ |
1611406434056011776 |