Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping

High-density whole-genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina® BeadStation was used to simul...

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Main Authors:	McKay, S D, Schnabel, R D, Murdoch, B M, Aerts, J, Gill, C A, Gao, C, Li, C, Matukumalli, L K, Stothard, P, Wang, Z, Van Tassell, C P, Williams, J L, Taylor, J F, Moore, S S
Format:	Online
Language:	English
Published:	Blackwell Publishing Ltd 2007
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063635/

id	pubmed-2063635
recordtype	oai_dc
spelling	pubmed-20636352007-11-06 Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping McKay, S D Schnabel, R D Murdoch, B M Aerts, J Gill, C A Gao, C Li, C Matukumalli, L K Stothard, P Wang, Z Van Tassell, C P Williams, J L Taylor, J F Moore, S S Original Articles High-density whole-genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina® BeadStation was used to simultaneously type large numbers of markers on the Roslin-Cambridge 3000 rad bovine–hamster whole-genome radiation hybrid panel (WGRH3000). In five multiplex reactions, 6738 sequence tagged site (STS) markers were successfully typed on the WGRH3000 panel DNA. These STSs harboured SNPs that were developed as a result of the bovine genome sequencing initiative. Typically, the most time consuming and expensive part of creating high-density radiation hybrid (RH) maps is genotyping the markers on the RH panel with conventional approaches. Using the method described in this article, we have developed a high-density whole-genome RH map with 4690 loci and a linkage map with 2701 loci, with direct comparison to the bovine whole-genome sequence assembly (Btau_2.0) in a fraction of the time it would have taken with conventional typing and genotyping methods. Blackwell Publishing Ltd 2007-04-01 /pmc/articles/PMC2063635/ /pubmed/17302794 http://dx.doi.org/10.1111/j.1365-2052.2006.01564.x Text en © 2007 The Authors, Journal compilation © 2007 International Society for Animal Genetics, Animal Genetics
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	McKay, S D Schnabel, R D Murdoch, B M Aerts, J Gill, C A Gao, C Li, C Matukumalli, L K Stothard, P Wang, Z Van Tassell, C P Williams, J L Taylor, J F Moore, S S
spellingShingle	McKay, S D Schnabel, R D Murdoch, B M Aerts, J Gill, C A Gao, C Li, C Matukumalli, L K Stothard, P Wang, Z Van Tassell, C P Williams, J L Taylor, J F Moore, S S Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
author_facet	McKay, S D Schnabel, R D Murdoch, B M Aerts, J Gill, C A Gao, C Li, C Matukumalli, L K Stothard, P Wang, Z Van Tassell, C P Williams, J L Taylor, J F Moore, S S
author_sort	McKay, S D
title	Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
title_short	Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
title_full	Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
title_fullStr	Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
title_full_unstemmed	Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
title_sort	construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping
description	High-density whole-genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina® BeadStation was used to simultaneously type large numbers of markers on the Roslin-Cambridge 3000 rad bovine–hamster whole-genome radiation hybrid panel (WGRH3000). In five multiplex reactions, 6738 sequence tagged site (STS) markers were successfully typed on the WGRH3000 panel DNA. These STSs harboured SNPs that were developed as a result of the bovine genome sequencing initiative. Typically, the most time consuming and expensive part of creating high-density radiation hybrid (RH) maps is genotyping the markers on the RH panel with conventional approaches. Using the method described in this article, we have developed a high-density whole-genome RH map with 4690 loci and a linkage map with 2701 loci, with direct comparison to the bovine whole-genome sequence assembly (Btau_2.0) in a fraction of the time it would have taken with conventional typing and genotyping methods.
publisher	Blackwell Publishing Ltd
publishDate	2007
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063635/
_version_	1611406178764455936

Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping

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