Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells
There have been a growing number of studies where molecular beacons (MBs) are used to image RNA expression in living cells; however, the ability to make accurate measurements can be hampered by the generation of false-positive signals resulting from non-specific interactions and/or nuclease degradat...
Main Authors: | , , |
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Format: | Online |
Language: | English |
Published: |
Oxford University Press
2007
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018645/ |